Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HVY04_RS18300 Genome accession   NZ_CP057509
Coordinates   3878579..3879559 (-) Length   326 a.a.
NCBI ID   WP_181819698.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHB24-C03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3873579..3884559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HVY04_RS18270 (HVY04_18275) - 3873828..3874703 (-) 876 WP_135911437.1 dihydrodipicolinate synthase family protein -
  HVY04_RS18275 (HVY04_18280) endA 3874941..3875648 (+) 708 WP_117342640.1 deoxyribonuclease I -
  HVY04_RS18280 (HVY04_18285) rsmE 3875723..3876454 (+) 732 WP_096755748.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HVY04_RS18285 (HVY04_18290) gshB 3876467..3877414 (+) 948 WP_181819697.1 glutathione synthase -
  HVY04_RS18290 (HVY04_18295) - 3877590..3878153 (+) 564 WP_096755746.1 YqgE/AlgH family protein -
  HVY04_RS18295 (HVY04_18300) ruvX 3878153..3878569 (+) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  HVY04_RS18300 (HVY04_18305) pilT 3878579..3879559 (-) 981 WP_181819698.1 type IV pilus twitching motility protein PilT Machinery gene
  HVY04_RS18305 (HVY04_18310) - 3879577..3880281 (+) 705 WP_096755743.1 YggS family pyridoxal phosphate-dependent enzyme -
  HVY04_RS18310 (HVY04_18315) - 3880300..3880866 (+) 567 WP_096755742.1 YggT family protein -
  HVY04_RS18315 (HVY04_18320) yggU 3880863..3881153 (+) 291 WP_003027101.1 DUF167 family protein YggU -
  HVY04_RS18320 (HVY04_18325) - 3881161..3881754 (+) 594 WP_115257510.1 XTP/dITP diphosphatase -
  HVY04_RS18325 (HVY04_18330) hemW 3881747..3882883 (+) 1137 WP_181540404.1 radical SAM family heme chaperone HemW -
  HVY04_RS18330 (HVY04_18335) ansB 3883008..3884054 (-) 1047 WP_096755739.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35743.98 Da        Isoelectric Point: 6.3354

>NTDB_id=461901 HVY04_RS18300 WP_181819698.1 3878579..3879559(-) (pilT) [Citrobacter freundii strain RHB24-C03]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
FCYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKIHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=461901 HVY04_RS18300 WP_181819698.1 3878579..3879559(-) (pilT) [Citrobacter freundii strain RHB24-C03]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAAGCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TTCTGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCACTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGTTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAATCCACCAGCTACCCGGCGTGATACAAACCGGACAACAGGCGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Legionella pneumophila strain ERS1305867

47.205

98.773

0.466
  pilT Legionella pneumophila strain Lp02

47.205

98.773

0.466
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.396

97.546

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

37.963

99.387

0.377