Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HVZ37_RS03750 Genome accession   NZ_CP057150
Coordinates   767429..768409 (+) Length   326 a.a.
NCBI ID   WP_061070563.1    Uniprot ID   A0AAW9M4G5
Organism   Citrobacter sp. RHB35-C17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 762429..773409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HVZ37_RS03720 (HVZ37_03725) - 763066..764073 (+) 1008 WP_109739853.1 DUF1202 family protein -
  HVZ37_RS03725 (HVZ37_03730) hemW 764105..765241 (-) 1137 WP_181628117.1 radical SAM family heme chaperone HemW -
  HVZ37_RS03730 (HVZ37_03735) - 765234..765827 (-) 594 WP_042998063.1 XTP/dITP diphosphatase -
  HVZ37_RS03735 (HVZ37_03740) yggU 765835..766125 (-) 291 WP_181628118.1 DUF167 family protein YggU -
  HVZ37_RS03740 (HVZ37_03745) - 766122..766688 (-) 567 WP_042998061.1 YggT family protein -
  HVZ37_RS03745 (HVZ37_03750) - 766707..767411 (-) 705 WP_061070564.1 YggS family pyridoxal phosphate-dependent enzyme -
  HVZ37_RS03750 (HVZ37_03755) pilT 767429..768409 (+) 981 WP_061070563.1 type IV pilus twitching motility protein PilT Machinery gene
  HVZ37_RS03755 (HVZ37_03760) ruvX 768423..768839 (-) 417 WP_042998058.1 Holliday junction resolvase RuvX -
  HVZ37_RS03760 (HVZ37_03765) - 768839..769402 (-) 564 WP_042998057.1 YqgE/AlgH family protein -
  HVZ37_RS03765 (HVZ37_03770) gshB 769577..770524 (-) 948 WP_044255655.1 glutathione synthase -
  HVZ37_RS03770 (HVZ37_03775) rsmE 770537..771268 (-) 732 WP_042998055.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HVZ37_RS03775 (HVZ37_03780) endA 771348..772055 (-) 708 WP_181628458.1 deoxyribonuclease I -
  HVZ37_RS03780 (HVZ37_03785) - 772147..772647 (-) 501 WP_042998053.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35833.90 Da        Isoelectric Point: 7.0211

>NTDB_id=461377 HVZ37_RS03750 WP_061070563.1 767429..768409(+) (pilT) [Citrobacter sp. RHB35-C17]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMERAPFTATDITGLLADWLDEGQQAALRQNGQLDFAVSLADNQRLRA
SAFHQRQGTSLALRLLPTQCPTLETLGTPSVLPELLRSENGLILVTGATGSGKSTTLAAMVTWLNQHIDGHILTLEDPIE
YIYTSQRCLIQQREAGLHCASFATGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLEPDKQEGRVALYELLINTPAAGNLIREGKSHQLPHVIQTGQQMGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=461377 HVZ37_RS03750 WP_061070563.1 767429..768409(+) (pilT) [Citrobacter sp. RHB35-C17]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGAAAACGGGGACGCATGGAGCGTGCACCCTTTACCGCAACCGACATTACCGGACTGCTAGCGGACTGGCTGGATG
AGGGGCAACAGGCGGCGTTACGACAAAATGGGCAACTGGATTTCGCGGTTTCGCTCGCGGATAACCAGCGCCTGCGGGCC
AGCGCCTTTCACCAGCGGCAGGGGACGTCGCTGGCGCTACGGCTGCTGCCGACGCAATGCCCGACGCTGGAAACGCTGGG
GACGCCTTCTGTGCTGCCCGAACTGCTCCGCAGCGAGAATGGTTTGATTCTGGTGACTGGGGCGACAGGCAGCGGAAAAT
CAACTACCCTGGCGGCGATGGTCACGTGGCTGAATCAGCATATCGATGGACATATTCTGACGCTGGAAGATCCCATCGAG
TATATCTACACCAGCCAGCGCTGCCTGATTCAGCAGCGTGAAGCAGGACTGCACTGCGCCTCATTTGCCACCGGTTTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGAGAGCTACGCGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACACGCGGTGCCGCGCAGGCGATCGAGCGACTGATTGATTCT
TTTCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCAGGCAGTCTGCGCGCCGTACTGTCGCAGAAACTGGAGCC
CGACAAGCAGGAGGGGCGCGTTGCGCTGTATGAACTGCTGATCAACACGCCGGCAGCGGGGAATTTGATTCGGGAAGGGA
AGTCCCATCAGTTACCCCATGTCATTCAGACCGGGCAGCAGATGGGGATGATGACCTTTGCCCAGAGCCTGCAACAGCGA
CAGGCACAAGGACGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.902

97.239

0.417
  pilU Vibrio cholerae strain A1552

42.581

95.092

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Acinetobacter baylyi ADP1

39.077

99.693

0.39