Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV011_RS03525 Genome accession   NZ_CP056967
Coordinates   719985..720965 (+) Length   326 a.a.
NCBI ID   WP_181596242.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHB41-C16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714985..725965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV011_RS03495 (HV011_03500) ansB 715490..716536 (+) 1047 WP_181596241.1 L-asparaginase 2 -
  HV011_RS03500 (HV011_03505) hemW 716661..717797 (-) 1137 WP_181540404.1 radical SAM family heme chaperone HemW -
  HV011_RS03505 (HV011_03510) - 717790..718383 (-) 594 WP_115257510.1 XTP/dITP diphosphatase -
  HV011_RS03510 (HV011_03515) yggU 718391..718681 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  HV011_RS03515 (HV011_03520) - 718678..719244 (-) 567 WP_096755742.1 YggT family protein -
  HV011_RS03520 (HV011_03525) - 719263..719967 (-) 705 WP_137399020.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV011_RS03525 (HV011_03530) pilT 719985..720965 (+) 981 WP_181596242.1 type IV pilus twitching motility protein PilT Machinery gene
  HV011_RS03530 (HV011_03535) ruvX 720975..721391 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  HV011_RS03535 (HV011_03540) - 721391..721954 (-) 564 WP_117342643.1 YqgE/AlgH family protein -
  HV011_RS03540 (HV011_03545) gshB 722130..723077 (-) 948 WP_117342642.1 glutathione synthase -
  HV011_RS03545 (HV011_03550) rsmE 723090..723821 (-) 732 WP_096755748.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV011_RS03550 (HV011_03555) endA 723896..724603 (-) 708 WP_181596243.1 deoxyribonuclease I -
  HV011_RS03555 (HV011_03560) - 724841..725716 (+) 876 WP_181596244.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35813.03 Da        Isoelectric Point: 6.6536

>NTDB_id=461106 HV011_RS03525 WP_181596242.1 719985..720965(+) (pilT) [Citrobacter freundii strain RHB41-C16]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHRQGTSLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
FRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=461106 HV011_RS03525 WP_181596242.1 719985..720965(+) (pilT) [Citrobacter freundii strain RHB41-C16]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAAGTTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCGGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TTCCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCACTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGTTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAGGCGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Legionella pneumophila strain ERS1305867

46.894

98.773

0.463
  pilT Legionella pneumophila strain Lp02

46.894

98.773

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.025

97.546

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

37.963

99.387

0.377