Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLO1313_RS05625 Genome accession   NC_017304
Coordinates   1323090..1324145 (+) Length   351 a.a.
NCBI ID   WP_003515764.1    Uniprot ID   A0AB36TGQ8
Organism   Acetivibrio thermocellus DSM 1313     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1315468..1361433 1323090..1324145 within 0


Gene organization within MGE regions


Location: 1315468..1361433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLO1313_RS05590 (Clo1313_1101) arsB 1315496..1316533 (+) 1038 WP_095522197.1 ACR3 family arsenite efflux transporter -
  CLO1313_RS05595 (Clo1313_1102) - 1316558..1316959 (+) 402 WP_003519126.1 arsenate reductase ArsC -
  CLO1313_RS05600 (Clo1313_1103) - 1317068..1317922 (+) 855 WP_003519124.1 permease -
  CLO1313_RS05610 (Clo1313_1104) - 1318796..1319948 (+) 1153 WP_011838186.1 IS3-like element IS120 family transposase -
  CLO1313_RS15895 (Clo1313_1105) - 1320224..1320385 (+) 162 WP_157881697.1 hypothetical protein -
  CLO1313_RS05620 (Clo1313_1106) - 1320711..1323074 (+) 2364 WP_003515767.1 type II/IV secretion system protein -
  CLO1313_RS05625 (Clo1313_1107) pilT 1323090..1324145 (+) 1056 WP_003515764.1 type IV pilus twitching motility protein PilT Machinery gene
  CLO1313_RS05630 (Clo1313_1108) - 1324604..1325818 (+) 1215 WP_003515762.1 type II secretion system F family protein -
  CLO1313_RS05635 (Clo1313_1109) - 1325973..1326479 (+) 507 WP_003515760.1 type II secretion system protein -
  CLO1313_RS05640 (Clo1313_1110) - 1326587..1327147 (+) 561 WP_003515758.1 type II secretion system protein J -
  CLO1313_RS05645 (Clo1313_1111) - 1327188..1327739 (+) 552 WP_003515756.1 PilN domain-containing protein -
  CLO1313_RS05650 (Clo1313_1112) - 1327739..1329031 (+) 1293 WP_003515754.1 hypothetical protein -
  CLO1313_RS05655 (Clo1313_1113) - 1329077..1329598 (+) 522 WP_003515752.1 type II secretion system protein -
  CLO1313_RS05660 (Clo1313_1114) - 1329675..1332224 (+) 2550 WP_003515750.1 pilus assembly PilX N-terminal domain-containing protein -
  CLO1313_RS05665 (Clo1313_1115) - 1332252..1333541 (+) 1290 WP_003521255.1 hypothetical protein -
  CLO1313_RS05670 (Clo1313_1116) - 1333692..1334465 (+) 774 WP_003515746.1 ClpP family protease -
  CLO1313_RS05675 (Clo1313_1117) - 1334462..1334686 (+) 225 WP_003515744.1 YlzJ-like family protein -
  CLO1313_RS05680 (Clo1313_1118) - 1334860..1337286 (+) 2427 WP_003521254.1 DNA translocase FtsK -
  CLO1313_RS05685 (Clo1313_1119) - 1337378..1339297 (+) 1920 WP_003515738.1 YgiQ family radical SAM protein -
  CLO1313_RS05690 (Clo1313_1120) folD 1339406..1340263 (+) 858 WP_003515736.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
  CLO1313_RS05695 (Clo1313_1121) - 1340358..1341035 (+) 678 WP_003515733.1 hypothetical protein -
  CLO1313_RS05700 (Clo1313_1122) rny 1341153..1342727 (+) 1575 WP_003515732.1 ribonuclease Y -
  CLO1313_RS05705 (Clo1313_1123) - 1342852..1343634 (+) 783 WP_003515729.1 TIGR00282 family metallophosphoesterase -
  CLO1313_RS05710 (Clo1313_1124) - 1343800..1344066 (+) 267 WP_003515727.1 stage V sporulation protein S -
  CLO1313_RS05715 (Clo1313_1125) - 1344338..1345078 (+) 741 WP_003515725.1 hypothetical protein -
  CLO1313_RS05720 (Clo1313_1126) - 1345147..1345410 (+) 264 WP_003515723.1 DUF3343 domain-containing protein -
  CLO1313_RS05725 (Clo1313_1127) - 1345488..1346474 (-) 987 WP_003515721.1 dTDP-glucose 4,6-dehydratase -
  CLO1313_RS05730 (Clo1313_1128) - 1346546..1347838 (-) 1293 WP_003515719.1 glycosyltransferase family 4 protein -
  CLO1313_RS05735 (Clo1313_1129) - 1347855..1348892 (-) 1038 WP_003515717.1 CotS family spore coat protein -
  CLO1313_RS05740 (Clo1313_1130) - 1348898..1349959 (-) 1062 WP_235715137.1 CotS family spore coat protein -
  CLO1313_RS05745 (Clo1313_1131) - 1350062..1350319 (-) 258 WP_003515712.1 amphi-Trp domain-containing protein -
  CLO1313_RS05750 (Clo1313_1132) - 1350483..1350950 (-) 468 WP_003515710.1 hypothetical protein -
  CLO1313_RS05755 (Clo1313_1133) - 1351160..1351483 (-) 324 WP_003515708.1 carbohydrate-binding protein -
  CLO1313_RS05760 (Clo1313_1134) - 1351854..1354316 (+) 2463 WP_003515705.1 mannose-1-phosphate guanyltransferase -
  CLO1313_RS05765 (Clo1313_1135) - 1354376..1354693 (-) 318 WP_003515703.1 hypothetical protein -
  CLO1313_RS05770 (Clo1313_1136) - 1354830..1355459 (-) 630 WP_003515701.1 hypothetical protein -
  CLO1313_RS05775 (Clo1313_1137) - 1355689..1356402 (+) 714 WP_014522613.1 DUF881 domain-containing protein -
  CLO1313_RS05780 (Clo1313_1138) - 1356402..1357103 (+) 702 WP_003515698.1 DUF881 domain-containing protein -
  CLO1313_RS05785 (Clo1313_1139) pdaA 1357336..1358298 (+) 963 WP_003515696.1 delta-lactam-biosynthetic de-N-acetylase -
  CLO1313_RS05790 (Clo1313_1140) yqfC 1358394..1358711 (+) 318 WP_003515694.1 sporulation protein YqfC -
  CLO1313_RS05795 (Clo1313_1141) yqfD 1358727..1359923 (+) 1197 WP_003515692.1 sporulation protein YqfD -
  CLO1313_RS05800 (Clo1313_1142) - 1359924..1360925 (+) 1002 WP_003515690.1 PhoH family protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38906.78 Da        Isoelectric Point: 6.9405

>NTDB_id=46043 CLO1313_RS05625 WP_003515764.1 1323090..1324145(+) (pilT) [Acetivibrio thermocellus DSM 1313]
MDINDLLRKTVENDASDLHICAGVPPIIRVYGDLINLNEPVLTPHDCVQLAKQCLSSRMYERFLEVGEVDASYSVPGVAR
FRVNVFKQRGTCAIAFRAIPTEVPSIEQLGLPGLVYDLAMKQRGLVLVTGPTGHGKSTTLAAMINYINSKRRCHIVTIED
PIEYLHRHNKSIINQREIGSDTKSYANALRAVLREDPDVILIGEMRDQETIATALTAAETGHLVLSTLHTVGAAKTIDRI
IDVFPPHQQSQVRVQLSTVLMGIISQQLIKRANGKGRVLATETMVWTPAISNLIRESRTPQINTCIQTGSQFGMYTMDSC
LAELYKAGEIDYESACQYSVDMDNLRKLISN

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=46043 CLO1313_RS05625 WP_003515764.1 1323090..1324145(+) (pilT) [Acetivibrio thermocellus DSM 1313]
ATGGATATAAATGATTTACTGAGGAAGACAGTCGAAAATGATGCGTCCGACTTGCATATTTGTGCGGGGGTGCCTCCGAT
TATAAGGGTCTACGGCGATCTTATAAATTTAAACGAGCCGGTGTTAACTCCGCATGATTGTGTGCAGCTTGCCAAGCAAT
GTTTAAGCAGCAGAATGTATGAACGCTTTCTTGAAGTCGGAGAAGTCGATGCATCGTACTCGGTTCCGGGAGTGGCACGA
TTCAGGGTAAATGTTTTCAAGCAAAGAGGTACTTGTGCAATTGCATTCAGAGCAATACCTACAGAAGTTCCGTCAATTGA
GCAGCTGGGGCTTCCCGGCTTGGTATATGACCTTGCCATGAAGCAGAGAGGTCTTGTTTTGGTAACGGGACCTACAGGTC
ATGGAAAGTCCACTACACTGGCTGCCATGATTAATTACATAAACAGCAAGAGAAGATGTCACATAGTTACGATTGAAGAC
CCTATAGAGTATTTGCACAGACATAACAAAAGTATTATAAATCAGAGGGAAATAGGTTCTGATACGAAATCCTACGCAAA
TGCGTTGAGAGCTGTATTAAGAGAAGACCCTGACGTTATACTTATCGGAGAGATGCGTGACCAGGAGACCATTGCAACGG
CGCTTACGGCTGCTGAAACAGGTCACTTGGTTTTGTCTACTTTGCATACCGTCGGAGCAGCAAAGACCATCGACAGAATT
ATCGACGTTTTCCCGCCTCACCAGCAGTCACAGGTAAGGGTTCAGCTTTCCACCGTGCTTATGGGTATCATATCCCAGCA
GCTTATAAAAAGAGCAAATGGCAAGGGACGTGTGCTGGCTACTGAGACCATGGTTTGGACGCCCGCTATTTCAAATCTTA
TTCGTGAAAGCCGTACACCTCAGATAAATACTTGTATTCAGACGGGTTCTCAGTTTGGAATGTATACCATGGACAGTTGC
CTTGCAGAACTCTATAAAGCCGGTGAAATTGACTATGAAAGTGCATGCCAGTATTCTGTTGATATGGATAATTTGAGAAA
ATTAATCTCAAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.187

97.721

0.51
  pilT Vibrio cholerae strain A1552

52.187

97.721

0.51
  pilT Neisseria meningitidis 8013

52.679

95.726

0.504
  pilT Neisseria gonorrhoeae MS11

52.381

95.726

0.501
  pilT Acinetobacter nosocomialis M2

52.537

95.442

0.501
  pilT Acinetobacter baumannii D1279779

52.537

95.442

0.501
  pilT Acinetobacter baumannii strain A118

52.537

95.442

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

100

0.499
  pilT Pseudomonas stutzeri DSM 10701

50.725

98.291

0.499
  pilT Legionella pneumophila strain ERS1305867

51.32

97.151

0.499
  pilT Legionella pneumophila strain Lp02

51.32

97.151

0.499
  pilT Pseudomonas aeruginosa PAK

52.239

95.442

0.499
  pilT Acinetobacter baylyi ADP1

52.239

95.442

0.499
  pilU Pseudomonas stutzeri DSM 10701

42.407

99.43

0.422
  pilU Acinetobacter baylyi ADP1

40.346

98.86

0.399
  pilU Vibrio cholerae strain A1552

39.143

99.715

0.39

Multiple sequence alignment