Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H04402_RS12550 Genome accession   NC_017299
Coordinates   2691503..2692549 (-) Length   348 a.a.
NCBI ID   WP_014521296.1    Uniprot ID   -
Organism   Clostridium botulinum H04402 065     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2686503..2697549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H04402_RS12530 (H04402_02567) ftsZ 2687019..2688128 (-) 1110 WP_014521294.1 cell division protein FtsZ -
  H04402_RS12535 (H04402_02568) ftsA 2688151..2689404 (-) 1254 WP_003386375.1 cell division protein FtsA -
  H04402_RS12540 (H04402_02569) - 2689562..2690836 (-) 1275 WP_014521295.1 glycosyltransferase family 2 protein -
  H04402_RS19955 - 2691275..2691418 (+) 144 WP_003386378.1 hypothetical protein -
  H04402_RS12550 (H04402_02570) pilT 2691503..2692549 (-) 1047 WP_014521296.1 type IV pilus twitching motility protein PilT Machinery gene
  H04402_RS12555 (H04402_02572) aroE 2692551..2693328 (-) 778 Protein_2435 shikimate dehydrogenase -
  H04402_RS12560 (H04402_02573) - 2693467..2693751 (-) 285 Protein_2436 sigma factor-like helix-turn-helix DNA-binding protein -
  H04402_RS12570 (H04402_02575) - 2694257..2694490 (+) 234 WP_014521301.1 helix-turn-helix transcriptional regulator -
  H04402_RS12575 (H04402_02576) - 2694565..2694843 (+) 279 WP_014521302.1 helix-turn-helix transcriptional regulator -
  H04402_RS12580 (H04402_02577) - 2694930..2695238 (+) 309 WP_014520407.1 helix-turn-helix domain-containing protein -
  H04402_RS19060 (H04402_02578) - 2695374..2695589 (+) 216 WP_148265697.1 IS3 family transposase -
  H04402_RS19065 (H04402_02579) - 2695667..2695981 (+) 315 WP_014521304.1 hypothetical protein -
  H04402_RS12590 (H04402_02580) - 2696194..2696439 (-) 246 WP_014521305.1 hypothetical protein -
  H04402_RS12595 (H04402_02581) - 2696456..2696644 (-) 189 WP_014521306.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38828.73 Da        Isoelectric Point: 8.0876

>NTDB_id=46018 H04402_RS12550 WP_014521296.1 2691503..2692549(-) (pilT) [Clostridium botulinum H04402 065]
MKQLKELLEKTINFGASDLHLSVGVEPTIRINGQLSKIFKEKLTPEDTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKNN

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=46018 H04402_RS12550 WP_014521296.1 2691503..2692549(-) (pilT) [Clostridium botulinum H04402 065]
TTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGTGCTTCAGATTTACATTTATCCGTAGGGGTAGAACC
AACTATAAGAATAAATGGACAATTAAGTAAAATATTTAAAGAAAAGCTTACACCAGAAGATACTAATAATTATGCCAAAG
AAATATTAGGTGAAGAATTTGAAGATTTATTGAAAAAAGGAGAAATAGATACATCCTATTCTATATCGGGCCTAGGTAGA
TTCAGAGTAAGCGTGTTTAAACAAAGAGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTGAAGATCCCTAACTTAAA
TGAACTAAAATTGCCTGATATAGTAAAGGAATTAACCAATAAAAAAAGAGGCCTTATTTTAGTAACGGGTCCTACAGGTT
CAGGCAAAAGCACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACTTTAGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGAGAGATAGGTAGGGATACTTTTAGTTATAAAAG
TGCTTTAAAAGCAGCATTAAGAGAAGATCCGGATGTAATATTAATAGGAGAGCTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGACATTTAGTATTTTCTACTTTGCATACTATAGGAGGGGCTAAAACTATAGATAGAATT
GTTAATGTATTTCCGCCACATCAACAGGAGCAGATAAGAATACAAGTAGCAAGTGTGTTAGAAGGAATAATAAGTCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCAGCCTTAGAGACTATGGTAACTACAAATGCCATAAGAAATATGATAA
GGGAAGGAAAAACTTATCAAATAGAATCTTCCATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCCTTA
GCAGAGCTATATAAAAGAGAAATTATAAGCTACGATACTGCATTAAATTATTCAGTAGATAAGGATTTAATTACAAAAAA
TAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.751

95.69

0.486
  pilT Legionella pneumophila strain Lp02

48.696

99.138

0.483
  pilT Legionella pneumophila strain ERS1305867

48.696

99.138

0.483
  pilT Pseudomonas aeruginosa PAK

49.405

96.552

0.477
  pilT Acinetobacter nosocomialis M2

49.55

95.69

0.474
  pilT Acinetobacter baumannii strain A118

49.55

95.69

0.474
  pilT Acinetobacter baumannii D1279779

49.55

95.69

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

94.828

0.474
  pilT Vibrio cholerae strain A1552

50

94.828

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.81

96.552

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.198

97.414

0.46
  pilT Neisseria gonorrhoeae MS11

47.463

96.264

0.457
  pilT Neisseria meningitidis 8013

47.463

96.264

0.457
  pilU Acinetobacter baylyi ADP1

41.124

97.126

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.126

0.397
  pilU Vibrio cholerae strain A1552

38.507

96.264

0.371

Multiple sequence alignment