Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV135_RS20500 Genome accession   NZ_CP056647
Coordinates   4249548..4250528 (-) Length   326 a.a.
NCBI ID   WP_141875633.1    Uniprot ID   -
Organism   Citrobacter sp. RHBSTW-00229     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4244548..4255528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV135_RS20470 (HV135_20490) - 4245329..4245826 (+) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -
  HV135_RS20475 (HV135_20495) endA 4245921..4246628 (+) 708 WP_016154392.1 deoxyribonuclease I -
  HV135_RS20480 (HV135_20500) rsmE 4246704..4247435 (+) 732 WP_181220827.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV135_RS20485 (HV135_20505) gshB 4247448..4248395 (+) 948 WP_016154394.1 glutathione synthase -
  HV135_RS20490 (HV135_20510) - 4248572..4249135 (+) 564 WP_016154395.1 YqgE/AlgH family protein -
  HV135_RS20495 (HV135_20515) ruvX 4249135..4249551 (+) 417 WP_181220829.1 Holliday junction resolvase RuvX -
  HV135_RS20500 (HV135_20520) pilT 4249548..4250528 (-) 981 WP_141875633.1 type IV pilus twitching motility protein PilT Machinery gene
  HV135_RS20505 (HV135_20525) - 4250546..4250716 (+) 171 Protein_4026 YggS family pyridoxal phosphate enzyme -
  HV135_RS20510 (HV135_20530) - 4250767..4251141 (-) 375 WP_181220830.1 hypothetical protein -
  HV135_RS20515 (HV135_20535) - 4251658..4251828 (-) 171 Protein_4028 type IV pili twitching motility protein PilT -
  HV135_RS20520 (HV135_20540) - 4251801..4252505 (+) 705 WP_181220831.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV135_RS20525 (HV135_20545) - 4252524..4253090 (+) 567 WP_016154401.1 YggT family protein -
  HV135_RS20530 (HV135_20550) yggU 4253087..4253377 (+) 291 WP_005123286.1 DUF167 family protein YggU -
  HV135_RS20535 (HV135_20555) - 4253385..4253978 (+) 594 WP_016154402.1 XTP/dITP diphosphatase -
  HV135_RS20540 (HV135_20560) hemW 4253971..4255107 (+) 1137 WP_019077663.1 radical SAM family heme chaperone HemW -
  HV135_RS26125 - 4255133..4255245 (-) 113 Protein_4034 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36010.16 Da        Isoelectric Point: 6.4975

>NTDB_id=460087 HV135_RS20500 WP_141875633.1 4249548..4250528(-) (pilT) [Citrobacter sp. RHBSTW-00229]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTPPDVEDLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPKLLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=460087 HV135_RS20500 WP_141875633.1 4249548..4250528(-) (pilT) [Citrobacter sp. RHBSTW-00229]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGGATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACCCCCGAGGCCATACCTAAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACTACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCACATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAACGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(69-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.42

99.387

0.362