Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV139_RS03885 Genome accession   NZ_CP056644
Coordinates   799002..799982 (+) Length   326 a.a.
NCBI ID   WP_181630228.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-00267     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 794002..804982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV139_RS03855 (HV139_03855) ansB 794507..795553 (+) 1047 WP_096755739.1 L-asparaginase 2 -
  HV139_RS03860 (HV139_03860) hemW 795678..796814 (-) 1137 WP_181630226.1 radical SAM family heme chaperone HemW -
  HV139_RS03865 (HV139_03865) - 796807..797400 (-) 594 WP_137362190.1 XTP/dITP diphosphatase -
  HV139_RS03870 (HV139_03870) yggU 797408..797698 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  HV139_RS03875 (HV139_03875) - 797695..798261 (-) 567 WP_096755742.1 YggT family protein -
  HV139_RS03880 (HV139_03880) - 798280..798984 (-) 705 WP_137399020.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV139_RS03885 (HV139_03885) pilT 799002..799982 (+) 981 WP_181630228.1 type IV pilus twitching motility protein PilT Machinery gene
  HV139_RS03890 (HV139_03890) ruvX 799992..800408 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  HV139_RS03895 (HV139_03895) - 800408..800971 (-) 564 WP_096755746.1 YqgE/AlgH family protein -
  HV139_RS03900 (HV139_03900) gshB 801147..802094 (-) 948 WP_117342642.1 glutathione synthase -
  HV139_RS03905 (HV139_03905) rsmE 802107..802838 (-) 732 WP_181630230.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV139_RS03910 (HV139_03910) endA 802913..803620 (-) 708 WP_115257516.1 deoxyribonuclease I -
  HV139_RS03915 (HV139_03915) - 803858..804732 (+) 875 Protein_768 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35831.99 Da        Isoelectric Point: 6.4952

>NTDB_id=460050 HV139_RS03885 WP_181630228.1 799002..799982(+) (pilT) [Citrobacter freundii strain RHBSTW-00267]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLQLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
YRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=460050 HV139_RS03885 WP_181630228.1 799002..799982(+) (pilT) [Citrobacter freundii strain RHBSTW-00267]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAAGCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCAGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TACCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCCGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCACTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGCTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAGGCGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.343

100

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.082

97.546

0.42
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371