Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV186_RS03670 Genome accession   NZ_CP056559
Coordinates   737395..738381 (+) Length   328 a.a.
NCBI ID   WP_181490971.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-00408     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 732395..743381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV186_RS03640 (HV186_03640) ansB 732900..733946 (+) 1047 WP_096755739.1 L-asparaginase 2 -
  HV186_RS03645 (HV186_03645) hemW 734071..735207 (-) 1137 WP_181490969.1 radical SAM family heme chaperone HemW -
  HV186_RS03650 (HV186_03650) - 735200..735793 (-) 594 WP_115257510.1 XTP/dITP diphosphatase -
  HV186_RS03655 (HV186_03655) yggU 735801..736091 (-) 291 WP_181490970.1 DUF167 family protein YggU -
  HV186_RS03660 (HV186_03660) - 736088..736654 (-) 567 WP_096755742.1 YggT family protein -
  HV186_RS03665 (HV186_03665) - 736673..737377 (-) 705 WP_096755743.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV186_RS03670 (HV186_03670) pilT 737395..738381 (+) 987 WP_181490971.1 type IV pilus twitching motility protein PilT Machinery gene
  HV186_RS03675 (HV186_03675) ruvX 738385..738801 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  HV186_RS03680 (HV186_03680) - 738801..739364 (-) 564 WP_096755746.1 YqgE/AlgH family protein -
  HV186_RS03685 (HV186_03685) gshB 739540..740487 (-) 948 WP_117342642.1 glutathione synthase -
  HV186_RS03690 (HV186_03690) rsmE 740500..741231 (-) 732 WP_181490972.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV186_RS03695 (HV186_03695) endA 741306..742013 (-) 708 WP_115257516.1 deoxyribonuclease I -
  HV186_RS03700 (HV186_03700) - 742251..743126 (+) 876 WP_181490973.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 328 a.a.        Molecular weight: 36042.26 Da        Isoelectric Point: 6.4952

>NTDB_id=459865 HV186_RS03670 WP_181490971.1 737395..738381(+) (pilT) [Citrobacter freundii strain RHBSTW-00408]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
YRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRLQI

Nucleotide


Download         Length: 987 bp        

>NTDB_id=459865 HV186_RS03670 WP_181490971.1 737395..738381(+) (pilT) [Citrobacter freundii strain RHBSTW-00408]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAAGCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TACCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCCGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCACTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGCTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAGGCGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGCAAATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

99.695

0.497
  pilT Vibrio cholerae strain A1552

49.847

99.695

0.497
  pilT Neisseria meningitidis 8013

46.951

100

0.47
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.584

98.171

0.457
  pilT Legionella pneumophila strain Lp02

46.584

98.171

0.457
  pilT Acinetobacter baylyi ADP1

45.015

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

99.695

0.451
  pilT Acinetobacter baumannii D1279779

45.26

99.695

0.451
  pilT Acinetobacter nosocomialis M2

44.954

99.695

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.954

99.695

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

99.695

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.396

96.951

0.421
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.384
  pilU Vibrio cholerae strain A1552

38.82

98.171

0.381
  pilU Acinetobacter baylyi ADP1

37.346

98.78

0.369