Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV232_RS19095 Genome accession   NZ_CP056409
Coordinates   4012924..4013904 (-) Length   326 a.a.
NCBI ID   WP_094168638.1    Uniprot ID   -
Organism   Citrobacter sp. RHBSTW-00535     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4007924..4018904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV232_RS19065 - 4008705..4009202 (+) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -
  HV232_RS19070 endA 4009297..4010004 (+) 708 WP_047357836.1 deoxyribonuclease I -
  HV232_RS19075 rsmE 4010080..4010811 (+) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV232_RS19080 gshB 4010824..4011771 (+) 948 WP_016154394.1 glutathione synthase -
  HV232_RS19085 - 4011948..4012511 (+) 564 WP_016154395.1 YqgE/AlgH family protein -
  HV232_RS19090 ruvX 4012511..4012927 (+) 417 WP_094168637.1 Holliday junction resolvase RuvX -
  HV232_RS19095 pilT 4012924..4013904 (-) 981 WP_094168638.1 type IV pilus twitching motility protein PilT Machinery gene
  HV232_RS19100 - 4013922..4014626 (+) 705 WP_094168639.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV232_RS19105 - 4014645..4015211 (+) 567 WP_016154401.1 YggT family protein -
  HV232_RS19110 yggU 4015208..4015498 (+) 291 WP_094168640.1 DUF167 family protein YggU -
  HV232_RS19115 - 4015506..4016099 (+) 594 WP_019077664.1 XTP/dITP diphosphatase -
  HV232_RS19120 hemW 4016092..4017228 (+) 1137 WP_019077663.1 radical SAM family heme chaperone HemW -
  HV232_RS19125 - 4017542..4018528 (+) 987 WP_047357840.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35896.96 Da        Isoelectric Point: 6.1727

>NTDB_id=459508 HV232_RS19095 WP_094168638.1 4012924..4013904(-) (pilT) [Citrobacter sp. RHBSTW-00535]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=459508 HV232_RS19095 WP_094168638.1 4012924..4013904(-) (pilT) [Citrobacter sp. RHBSTW-00535]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTCCA
GACCCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATGTTGCGGGGCATATTCTGACTCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGTTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGTTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365