Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV271_RS03765 Genome accession   NZ_CP056333
Coordinates   758223..759203 (+) Length   326 a.a.
NCBI ID   WP_181540406.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-00714     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 753223..764203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV271_RS03735 (HV271_03735) ansB 753728..754774 (+) 1047 WP_096755739.1 L-asparaginase 2 -
  HV271_RS03740 (HV271_03740) hemW 754899..756035 (-) 1137 WP_181540404.1 radical SAM family heme chaperone HemW -
  HV271_RS03745 (HV271_03745) - 756028..756621 (-) 594 WP_181540405.1 XTP/dITP diphosphatase -
  HV271_RS03750 (HV271_03750) yggU 756629..756919 (-) 291 WP_115257511.1 DUF167 family protein YggU -
  HV271_RS03755 (HV271_03755) - 756916..757482 (-) 567 WP_096755742.1 YggT family protein -
  HV271_RS03760 (HV271_03760) - 757501..758205 (-) 705 WP_137399020.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV271_RS03765 (HV271_03765) pilT 758223..759203 (+) 981 WP_181540406.1 type IV pilus twitching motility protein PilT Machinery gene
  HV271_RS03770 (HV271_03770) ruvX 759213..759629 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  HV271_RS03775 (HV271_03775) - 759629..760192 (-) 564 WP_096755746.1 YqgE/AlgH family protein -
  HV271_RS03780 (HV271_03780) gshB 760368..761315 (-) 948 WP_117342642.1 glutathione synthase -
  HV271_RS03785 (HV271_03785) rsmE 761328..762059 (-) 732 WP_096755748.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV271_RS03790 (HV271_03790) endA 762134..762841 (-) 708 WP_181540407.1 deoxyribonuclease I -
  HV271_RS03795 (HV271_03795) - 763079..763954 (+) 876 WP_181540408.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35770.90 Da        Isoelectric Point: 6.3365

>NTDB_id=459219 HV271_RS03765 WP_181540406.1 758223..759203(+) (pilT) [Citrobacter freundii strain RHBSTW-00714]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVENLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
FRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=459219 HV271_RS03765 WP_181540406.1 758223..759203(+) (pilT) [Citrobacter freundii strain RHBSTW-00714]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAATCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TTCCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGTTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAAGCGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.396

97.546

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

37.654

99.387

0.374