Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV325_RS03785 Genome accession   NZ_CP056245
Coordinates   764527..765507 (+) Length   326 a.a.
NCBI ID   WP_049015286.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-00915     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 759527..770507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV325_RS03755 (HV325_03755) ansB 760041..761087 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  HV325_RS03760 (HV325_03760) hemW 761203..762339 (-) 1137 WP_049015277.1 radical SAM family heme chaperone HemW -
  HV325_RS03765 (HV325_03765) - 762332..762925 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  HV325_RS03770 (HV325_03770) yggU 762933..763223 (-) 291 WP_049015279.1 DUF167 family protein YggU -
  HV325_RS03775 (HV325_03775) - 763220..763786 (-) 567 WP_049015282.1 YggT family protein -
  HV325_RS03780 (HV325_03780) - 763805..764509 (-) 705 WP_049015284.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV325_RS03785 (HV325_03785) pilT 764527..765507 (+) 981 WP_049015286.1 type IV pilus twitching motility protein PilT Machinery gene
  HV325_RS03790 (HV325_03790) ruvX 765504..765920 (-) 417 WP_003027087.1 Holliday junction resolvase RuvX -
  HV325_RS03795 (HV325_03795) - 765920..766483 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  HV325_RS03800 (HV325_03800) gshB 766595..767542 (-) 948 WP_003027083.1 glutathione synthase -
  HV325_RS03805 (HV325_03805) rsmE 767562..768293 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV325_RS03810 (HV325_03810) endA 768368..769075 (-) 708 WP_016150969.1 deoxyribonuclease I -
  HV325_RS03815 (HV325_03815) - 769170..769667 (-) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35905.02 Da        Isoelectric Point: 6.3638

>NTDB_id=458890 HV325_RS03785 WP_049015286.1 764527..765507(+) (pilT) [Citrobacter freundii strain RHBSTW-00915]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRHGKVESAPFTAPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHLQGTSLALRLLPLDCPHLDDIQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYISRRCLIQQREVGAHCASFATGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=458890 HV325_RS03785 WP_049015286.1 764527..765507(+) (pilT) [Citrobacter freundii strain RHBSTW-00915]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCAGACATGGAAAAGTCGAAAGCGCACCGTTTACCGCGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCCCATTTAGACGATATTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAACGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACATCAGCCGACGTTGTCTAATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCACCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGAGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCACTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTACAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.342

98.773

0.448
  pilT Legionella pneumophila strain Lp02

45.342

98.773

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362