Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV327_RS03580 Genome accession   NZ_CP056244
Coordinates   730539..731519 (+) Length   326 a.a.
NCBI ID   WP_181517840.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-00923     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 725539..736519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV327_RS03550 (HV327_03550) ansB 726044..727090 (+) 1047 WP_096755739.1 L-asparaginase 2 -
  HV327_RS03555 (HV327_03555) hemW 727215..728351 (-) 1137 WP_135911432.1 radical SAM family heme chaperone HemW -
  HV327_RS03560 (HV327_03560) - 728344..728937 (-) 594 WP_181517839.1 XTP/dITP diphosphatase -
  HV327_RS03565 (HV327_03565) yggU 728945..729235 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  HV327_RS03570 (HV327_03570) - 729232..729798 (-) 567 WP_096755742.1 YggT family protein -
  HV327_RS03575 (HV327_03575) - 729817..730521 (-) 705 WP_137399020.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV327_RS03580 (HV327_03580) pilT 730539..731519 (+) 981 WP_181517840.1 type IV pilus twitching motility protein PilT Machinery gene
  HV327_RS03585 (HV327_03585) ruvX 731529..731945 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  HV327_RS03590 (HV327_03590) - 731945..732508 (-) 564 WP_117342643.1 YqgE/AlgH family protein -
  HV327_RS03595 (HV327_03595) gshB 732684..733631 (-) 948 WP_181517841.1 glutathione synthase -
  HV327_RS03600 (HV327_03600) rsmE 733644..734375 (-) 732 WP_135911436.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV327_RS03605 (HV327_03605) endA 734450..735157 (-) 708 WP_117342640.1 deoxyribonuclease I -
  HV327_RS03610 (HV327_03610) - 735395..736270 (+) 876 WP_181517842.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35765.93 Da        Isoelectric Point: 6.4234

>NTDB_id=458869 HV327_RS03580 WP_181517840.1 730539..731519(+) (pilT) [Citrobacter freundii strain RHBSTW-00923]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
FRYTSQHCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=458869 HV327_RS03580 WP_181517840.1 730539..731519(+) (pilT) [Citrobacter freundii strain RHBSTW-00923]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAAGCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTACTGCCGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TTCCGCTATACCAGCCAGCATTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCACTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGCTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAAGCGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Legionella pneumophila strain ERS1305867

46.894

98.773

0.463
  pilT Legionella pneumophila strain Lp02

46.894

98.773

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.711

97.546

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

37.654

99.387

0.374