Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV336_RS03910 Genome accession   NZ_CP056232
Coordinates   810244..811224 (+) Length   326 a.a.
NCBI ID   WP_181510262.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-00965     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 805244..816224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV336_RS03880 (HV336_03860) ansB 805757..806803 (+) 1047 WP_181510259.1 L-asparaginase 2 -
  HV336_RS03885 (HV336_03865) hemW 806920..808056 (-) 1137 WP_181510260.1 radical SAM family heme chaperone HemW -
  HV336_RS03890 (HV336_03870) - 808049..808642 (-) 594 WP_079938668.1 XTP/dITP diphosphatase -
  HV336_RS03895 (HV336_03875) yggU 808650..808940 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  HV336_RS03900 (HV336_03880) - 808937..809503 (-) 567 WP_003825417.1 YggT family protein -
  HV336_RS03905 (HV336_03885) - 809522..810226 (-) 705 WP_181510261.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV336_RS03910 (HV336_03890) pilT 810244..811224 (+) 981 WP_181510262.1 type IV pilus twitching motility protein PilT Machinery gene
  HV336_RS03915 (HV336_03895) ruvX 811221..811637 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  HV336_RS03920 (HV336_03900) - 811637..812200 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  HV336_RS03925 (HV336_03905) gshB 812377..813324 (-) 948 WP_181510263.1 glutathione synthase -
  HV336_RS03930 (HV336_03910) rsmE 813344..814075 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV336_RS03935 (HV336_03915) endA 814150..814857 (-) 708 WP_008785767.1 deoxyribonuclease I -
  HV336_RS03940 (HV336_03920) - 814952..815449 (-) 498 WP_181510264.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35887.95 Da        Isoelectric Point: 6.4960

>NTDB_id=458799 HV336_RS03910 WP_181510262.1 810244..811224(+) (pilT) [Citrobacter freundii strain RHBSTW-00965]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVETAPFTTPDVENLLMCWLSEQQQVQWREQGQVDFAIALAGSQRLRA
SAFAHQQGTSLALRLLPLNCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQQVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FSATEKEPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=458799 HV336_RS03910 WP_181510262.1 810244..811224(+) (pilT) [Citrobacter freundii strain RHBSTW-00965]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAACCGCACCATTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGGTAGATTTTGCTATTGCGCTGGCAGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGCTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTAATTGCCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTTGAGTACCTTAATCAGCAAGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGAGAGGTGGGCGTGCACTGCGCTTCTTTTGCCGCTGGTTTACG
CGGCGCGTTGCGCGAAGATCCCGACGTCATTTTGCTGGGTGAGCTGCGCGATGTGGAAACCATTCGTCTGGCGTTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCTGCGCAGGCTATCGCACGGCTGGTGGACACC
TTTTCGGCAACAGAGAAAGAGCCGGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGAAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACGGGCATGCAGACGTTTGCACAAAGTCAGCAGCAGCGT
CAGGCGCAGGGGCGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.201

100

0.466
  pilT Acinetobacter baylyi ADP1

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter baumannii strain A118

44.985

100

0.454
  pilT Acinetobacter baumannii D1279779

44.985

100

0.454
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Acinetobacter nosocomialis M2

44.681

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365