Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV346_RS18555 Genome accession   NZ_CP056199
Coordinates   3888218..3889201 (-) Length   327 a.a.
NCBI ID   WP_181620710.1    Uniprot ID   A0A7L6CPB8
Organism   Enterobacter sp. RHBSTW-00994     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3883218..3894201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV346_RS18520 (HV346_18525) - 3883289..3883786 (+) 498 WP_181620703.1 SprT family zinc-dependent metalloprotease -
  HV346_RS18525 (HV346_18530) endA 3883881..3884588 (+) 708 WP_181620704.1 deoxyribonuclease I -
  HV346_RS18530 (HV346_18535) rsmE 3884641..3885372 (+) 732 WP_181620705.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV346_RS18535 (HV346_18540) gshB 3885404..3886351 (+) 948 WP_181620706.1 glutathione synthase -
  HV346_RS18540 (HV346_18545) - 3886439..3886999 (+) 561 WP_181620707.1 YqgE/AlgH family protein -
  HV346_RS18545 (HV346_18550) ruvX 3886999..3887415 (+) 417 WP_181620708.1 Holliday junction resolvase RuvX -
  HV346_RS18550 (HV346_18555) - 3887381..3888130 (-) 750 WP_181620709.1 IclR family transcriptional regulator -
  HV346_RS18555 (HV346_18560) pilT 3888218..3889201 (-) 984 WP_181620710.1 type IV pilus twitching motility protein PilT Machinery gene
  HV346_RS18560 (HV346_18565) - 3889220..3889924 (+) 705 WP_181620711.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV346_RS18565 (HV346_18570) - 3889943..3890509 (+) 567 WP_181620712.1 YggT family protein -
  HV346_RS18570 (HV346_18575) yggU 3890506..3890802 (+) 297 WP_181620713.1 DUF167 family protein YggU -
  HV346_RS18575 (HV346_18580) - 3890806..3891399 (+) 594 WP_181620714.1 XTP/dITP diphosphatase -
  HV346_RS18580 (HV346_18585) hemW 3891392..3892540 (+) 1149 WP_181620715.1 radical SAM family heme chaperone HemW -
  HV346_RS18585 (HV346_18590) - 3892597..3892910 (+) 314 Protein_3634 DUF559 domain-containing protein -
  HV346_RS18590 (HV346_18595) - 3892974..3893693 (-) 720 WP_181620716.1 DUF2884 domain-containing protein -
  HV346_RS18595 (HV346_18600) - 3893747..3894073 (-) 327 WP_181620717.1 YggL family protein -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 35741.82 Da        Isoelectric Point: 6.3029

>NTDB_id=458654 HV346_RS18555 WP_181620710.1 3888218..3889201(-) (pilT) [Enterobacter sp. RHBSTW-00994]
MDMEEIVALSVKHNVSDLHLCSDSPPRWRRLGKLEPAPFPSPNVDALLKTWLTDEQQGIWWANGQVDFAVTLASQQRLRG
SAFVHINGVSMTLRLLPLACPVLSALGVPRALPELLSSDNGLILVTGATGSGKSTTLAAMVDYLNKHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSTSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQKLVRDVQGGRVALYELLVNTSAAANLIREGKTWQLPGVIQMGQQAGMQNFDQSQAER
RAQGRLQ

Nucleotide


Download         Length: 984 bp        

>NTDB_id=458654 HV346_RS18555 WP_181620710.1 3888218..3889201(-) (pilT) [Enterobacter sp. RHBSTW-00994]
ATGGATATGGAAGAAATTGTGGCGCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTGATTCCCCTCCGCG
ATGGAGGCGATTGGGCAAGCTGGAGCCTGCGCCATTCCCATCACCGAACGTTGACGCGCTATTGAAAACCTGGTTAACCG
ACGAGCAGCAGGGCATCTGGTGGGCGAATGGTCAGGTTGATTTTGCGGTTACGCTCGCCAGTCAACAGCGTTTGCGTGGC
AGTGCTTTTGTGCATATTAACGGTGTCTCAATGACTTTGCGGCTCTTGCCTCTTGCCTGCCCGGTATTGTCAGCGCTTGG
CGTACCACGTGCACTGCCCGAACTGTTATCCAGTGACAATGGCCTGATTCTGGTCACGGGGGCAACGGGGAGTGGTAAAT
CCACAACACTTGCCGCGATGGTGGACTACCTCAATAAACATGCCGATGGGCATATTCTGACACTGGAAGATCCCGTTGAG
TTTATTTATCAGAGTGAGCGTTGCCTGATTCAGCAGCGGGAGATCGGCCAGCACAGCACATCGTTTGCAGAGGCGTTGCG
CAGTGCGTTGCGAGAGGACCCGGATGTGATCTTACTCGGGGAACTGCGCGACAGCGAAACTATCCGTTTGGCACTCACGG
CGGCGGAGACAGGGCATCTGGTGTTGGCAACGCTGCATACGCGTGGGGCGTCTCAGGCCATTGAGCGTCTGGTCGATACC
TTCCCGGCGCAGGAAAAAGATCCAGTGCGCAGTCAGTTGGCGGGAAGTTTACGTGCGGTATTGGCGCAGAAGCTGGTTCG
CGATGTTCAGGGAGGGCGTGTTGCGCTGTACGAATTGTTAGTGAACACCTCAGCGGCAGCAAACCTGATTCGTGAGGGTA
AAACCTGGCAACTTCCCGGTGTTATTCAGATGGGGCAACAGGCGGGAATGCAAAATTTTGACCAAAGCCAGGCAGAACGC
CGGGCGCAGGGACGCCTTCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L6CPB8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.498
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.498
  pilT Neisseria meningitidis 8013

48.438

97.859

0.474
  pilT Legionella pneumophila strain ERS1305867

48.137

98.471

0.474
  pilT Legionella pneumophila strain Lp02

48.137

98.471

0.474
  pilT Neisseria gonorrhoeae MS11

48.125

97.859

0.471
  pilT Pseudomonas stutzeri DSM 10701

47.95

96.942

0.465
  pilT Acinetobacter baylyi ADP1

46.177

100

0.462
  pilT Pseudomonas aeruginosa PAK

47.003

96.942

0.456
  pilT Acinetobacter baumannii strain A118

46.708

97.554

0.456
  pilT Acinetobacter nosocomialis M2

46.708

97.554

0.456
  pilT Acinetobacter baumannii D1279779

46.708

97.554

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.248

0.404
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.401
  pilU Vibrio cholerae strain A1552

39.185

97.554

0.382
  pilU Acinetobacter baylyi ADP1

36.957

98.471

0.364