Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV351_RS21085 Genome accession   NZ_CP056181
Coordinates   4335936..4336916 (-) Length   326 a.a.
NCBI ID   WP_181641162.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-01016     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4330936..4341916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV351_RS21055 (HV351_21065) - 4331712..4332209 (+) 498 WP_003846365.1 SprT family zinc-dependent metalloprotease -
  HV351_RS21060 (HV351_21070) endA 4332304..4333011 (+) 708 WP_174328941.1 deoxyribonuclease I -
  HV351_RS21065 (HV351_21075) rsmE 4333086..4333817 (+) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV351_RS21070 (HV351_21080) gshB 4333837..4334784 (+) 948 WP_003027083.1 glutathione synthase -
  HV351_RS21075 (HV351_21085) - 4334960..4335523 (+) 564 WP_003027086.1 YqgE/AlgH family protein -
  HV351_RS21080 (HV351_21090) ruvX 4335523..4335939 (+) 417 WP_006686843.1 Holliday junction resolvase RuvX -
  HV351_RS21085 (HV351_21095) pilT 4335936..4336916 (-) 981 WP_181641162.1 type IV pilus twitching motility protein PilT Machinery gene
  HV351_RS21090 (HV351_21100) - 4336934..4337638 (+) 705 WP_003838215.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV351_RS21095 (HV351_21105) - 4337657..4338223 (+) 567 WP_174328939.1 YggT family protein -
  HV351_RS21100 (HV351_21110) yggU 4338220..4338510 (+) 291 WP_003027101.1 DUF167 family protein YggU -
  HV351_RS21105 (HV351_21115) - 4338518..4339111 (+) 594 WP_003027104.1 XTP/dITP diphosphatase -
  HV351_RS21110 (HV351_21120) hemW 4339104..4340240 (+) 1137 WP_174328938.1 radical SAM family heme chaperone HemW -
  HV351_RS21115 (HV351_21125) ansB 4340356..4341402 (-) 1047 WP_044713806.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35896.96 Da        Isoelectric Point: 6.4255

>NTDB_id=458588 HV351_RS21085 WP_181641162.1 4335936..4336916(-) (pilT) [Citrobacter freundii strain RHBSTW-01016]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKVEGAPFTTPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHQQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=458588 HV351_RS21085 WP_181641162.1 4335936..4336916(-) (pilT) [Citrobacter freundii strain RHBSTW-01016]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGGCGTGGAAAAGTCGAAGGCGCACCGTTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCAGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTACTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365