Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TCCBUS3UF1_RS10695 Genome accession   NC_017278
Coordinates   2119378..2120463 (+) Length   361 a.a.
NCBI ID   WP_014516521.1    Uniprot ID   -
Organism   Thermus sp. CCB_US3_UF1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2114378..2125463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TCCBUS3UF1_RS10670 (TCCBUS3UF1_21300) ribH 2114516..2114989 (+) 474 WP_014516516.1 6,7-dimethyl-8-ribityllumazine synthase -
  TCCBUS3UF1_RS10675 (TCCBUS3UF1_21310) - 2114976..2115380 (-) 405 WP_014516517.1 DUF4395 family protein -
  TCCBUS3UF1_RS10680 (TCCBUS3UF1_21320) pgeF 2115458..2116186 (+) 729 WP_014516518.1 peptidoglycan editing factor PgeF -
  TCCBUS3UF1_RS10685 (TCCBUS3UF1_21330) - 2116221..2116703 (+) 483 WP_014516519.1 YqeG family HAD IIIA-type phosphatase -
  TCCBUS3UF1_RS10690 (TCCBUS3UF1_21340) pilF 2116700..2119366 (+) 2667 WP_014516520.1 type IV pilus assembly ATPase PilB Machinery gene
  TCCBUS3UF1_RS10695 (TCCBUS3UF1_21350) pilT 2119378..2120463 (+) 1086 WP_014516521.1 type IV pilus twitching motility protein PilT Machinery gene
  TCCBUS3UF1_RS10700 (TCCBUS3UF1_21360) gatB 2120486..2121895 (+) 1410 WP_014516522.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  TCCBUS3UF1_RS10705 (TCCBUS3UF1_21370) purM 2121901..2122902 (+) 1002 WP_014516523.1 phosphoribosylformylglycinamidine cyclo-ligase -
  TCCBUS3UF1_RS10710 (TCCBUS3UF1_21380) - 2122899..2123528 (+) 630 WP_014516524.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39991.39 Da        Isoelectric Point: 6.9845

>NTDB_id=45836 TCCBUS3UF1_RS10695 WP_014516521.1 2119378..2120463(+) (pilT) [Thermus sp. CCB_US3_UF1]
MPKTPDIVDLLTLAVERGASDLVITVGLPPMIKVDGEFHPTEYEPLSPQDTRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPASIKSFEELGLPKAIAEIAMAPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHRHKKAIINQREIGADTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPEVQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQFGMIT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGVEEGTRR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=45836 TCCBUS3UF1_RS10695 WP_014516521.1 2119378..2120463(+) (pilT) [Thermus sp. CCB_US3_UF1]
ATGCCCAAAACCCCCGATATCGTAGACCTTTTGACCCTGGCCGTGGAGCGGGGAGCCAGCGACCTGGTGATCACCGTGGG
CCTTCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCTCCCCCCAGGACACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGATCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCG
GGCAAGGGCCGCTACCGGGTGAACGTCTTCCTGCAACGGGGGAGCGTGGGCGGGGTCCTGCGGGTGGTGCCGGCGTCCAT
CAAAAGCTTTGAGGAGCTGGGCCTGCCCAAGGCCATCGCCGAGATCGCCATGGCCCCCCGGGGCCTGGTCCTGGTCACCG
GGCCCACGGGAAGTGGCAAGAGCACCACCCTGGCCTCCATGATCGACTACATCAACGAGCGCAAGCCGGTGCACATCGTG
ACCATCGAGGACCCCATTGAGTTCTTCCACCGGCACAAGAAGGCCATCATCAACCAACGGGAGATCGGGGCCGACACCCA
CGGGTTCCACAAGGCCTTGCGGAGCGTCTTGCGCCAGGCTCCCGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACCGGCCACCTGGTCATGGGTACCCTGCACACCAACTCCGCCCCGGAGACG
ATTGACCGGATCATCGACGTCTTCCCCGAGGTCCAGCAGGAACAGGTCCGGGTCCAGCTCTCCAACAACCTGGTGGCCGT
CCTCACCCAGCAGCTCCTGCCCAAGGCCTTTGGAGGCGGGCGGGTCCTGGCCTATGAGCTCATGATCGCCACCCCGGCGG
TGCGGGCCCTGATCCGGGAGGGCAAAAGCCACCAGCTCCGAAGCGTAATCCAGACGGGAGGCCAGTTCGGCATGATCACC
ATGGACGCCTGCCTGGCCGACCTGTACAAGCGCAAGCTCATCACCTACGAGATGGGCCTGGCCCGGGCCGTGGACCCCAA
GGAGTTCATGCGCCTGGCGGGGGTGGAGGAGGGCACCCGGCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.678

96.399

0.662
  pilT Acinetobacter baumannii D1279779

54.493

95.568

0.521
  pilT Acinetobacter nosocomialis M2

54.493

95.568

0.521
  pilT Acinetobacter baumannii strain A118

54.493

95.568

0.521
  pilT Acinetobacter baylyi ADP1

54.651

95.291

0.521
  pilT Pseudomonas aeruginosa PAK

54.07

95.291

0.515
  pilT Pseudomonas stutzeri DSM 10701

53.779

95.291

0.512
  pilT Neisseria meningitidis 8013

52.367

93.629

0.49
  pilT Legionella pneumophila strain ERS1305867

51.453

95.291

0.49
  pilT Legionella pneumophila strain Lp02

51.453

95.291

0.49
  pilT Vibrio cholerae strain A1552

52.888

91.136

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.888

91.136

0.482
  pilT Neisseria gonorrhoeae MS11

51.632

93.352

0.482
  pilU Pseudomonas stutzeri DSM 10701

47.23

95.014

0.449
  pilU Vibrio cholerae strain A1552

44.611

92.521

0.413
  pilU Acinetobacter baylyi ADP1

42.319

95.568

0.404

Multiple sequence alignment