Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV119_RS04060 Genome accession   NZ_CP055551
Coordinates   874759..875739 (+) Length   326 a.a.
NCBI ID   WP_100194828.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHBSTW-00200     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869759..880739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV119_RS04030 (HV119_04030) ansB 870272..871318 (+) 1047 WP_043017547.1 L-asparaginase 2 -
  HV119_RS04035 (HV119_04035) hemW 871435..872571 (-) 1137 WP_087051205.1 radical SAM family heme chaperone HemW -
  HV119_RS04040 (HV119_04040) - 872564..873157 (-) 594 WP_047408837.1 XTP/dITP diphosphatase -
  HV119_RS04045 (HV119_04045) yggU 873165..873455 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  HV119_RS04050 (HV119_04050) - 873452..874018 (-) 567 WP_003825417.1 YggT family protein -
  HV119_RS04055 (HV119_04055) - 874037..874741 (-) 705 WP_181231088.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV119_RS04060 (HV119_04060) pilT 874759..875739 (+) 981 WP_100194828.1 type IV pilus twitching motility protein PilT Machinery gene
  HV119_RS04065 (HV119_04065) ruvX 875736..876152 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  HV119_RS04070 (HV119_04070) - 876152..876715 (-) 564 WP_047408844.1 YqgE/AlgH family protein -
  HV119_RS04075 (HV119_04075) gshB 876892..877839 (-) 948 WP_043017542.1 glutathione synthase -
  HV119_RS04080 (HV119_04080) rsmE 877859..878590 (-) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV119_RS04085 (HV119_04085) endA 878665..879372 (-) 708 WP_043017541.1 deoxyribonuclease I -
  HV119_RS04090 (HV119_04090) - 879467..879964 (-) 498 WP_181231089.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36030.33 Da        Isoelectric Point: 6.7175

>NTDB_id=457742 HV119_RS04060 WP_100194828.1 874759..875739(+) (pilT) [Citrobacter freundii strain RHBSTW-00200]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTPPDVENLLMCWLSEQQQVQWRKQGQIDFAIALADSRRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPEAIPELLHSEHGLILVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKLGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=457742 HV119_RS04060 WP_100194828.1 874759..875739(+) (pilT) [Citrobacter freundii strain RHBSTW-00200]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGTGGAAAAGTCGAAATCGCGCCATTTACTCCGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGCG
AGCAACAACAGGTACAGTGGCGGAAACAGGGGCAGATTGATTTTGCTATTGCGCTGGCAGATTCCCGACGTCTGCGCGCC
AGCGCTTTTGTTCATCAACAGGGTACGTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACTCCCGAGGCCATACCTGAACTGCTGCACAGTGAACATGGATTGATTCTGGTCACGGGAGCCACCGGCAGCGGTAAAT
CAACAACCCTGGCAGCGATGGTGGAGTACCTTAATCAGCATATTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGCTGTCTTATTCAACAACGGGAGGTGGGCGTTCACTGTGCTTCTTTTGCCGCTGGCTTGCG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGTGGAAACCATTCGTCTGGCATTAACGG
CAGCGGAAACCGGGCACCTGGTGCTGGCAACGTTACATACGCGAGGGGCTGCGCAGGCTATCGCACGGCTGGTGGATACC
TTTCCCGCGCAGGAGAAGGATCCGGTGCGTAATCAACTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAGGA
AGATAAGCTGGGAGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGCATGCAGACGTTTGCACAAAGTCTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368