Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV183_RS19910 Genome accession   NZ_CP055538
Coordinates   4160320..4161300 (-) Length   326 a.a.
NCBI ID   WP_181218114.1    Uniprot ID   A0AAE7L0A8
Organism   Citrobacter freundii strain RHBSTW-00398     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4155320..4166300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV183_RS19880 (HV183_19885) - 4155569..4156444 (-) 876 WP_181218112.1 dihydrodipicolinate synthase family protein -
  HV183_RS19885 (HV183_19890) endA 4156682..4157389 (+) 708 WP_115257516.1 deoxyribonuclease I -
  HV183_RS19890 (HV183_19895) rsmE 4157464..4158195 (+) 732 WP_096755748.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV183_RS19895 (HV183_19900) gshB 4158208..4159155 (+) 948 WP_117342642.1 glutathione synthase -
  HV183_RS19900 (HV183_19905) - 4159331..4159894 (+) 564 WP_096755746.1 YqgE/AlgH family protein -
  HV183_RS19905 (HV183_19910) ruvX 4159894..4160310 (+) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  HV183_RS19910 (HV183_19915) pilT 4160320..4161300 (-) 981 WP_181218114.1 type IV pilus twitching motility protein PilT Machinery gene
  HV183_RS19915 (HV183_19920) - 4161318..4162022 (+) 705 WP_181218116.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV183_RS19920 (HV183_19925) - 4162041..4162607 (+) 567 WP_181218117.1 YggT family protein -
  HV183_RS19925 (HV183_19930) yggU 4162604..4162894 (+) 291 WP_003027101.1 DUF167 family protein YggU -
  HV183_RS19930 (HV183_19935) - 4162902..4163495 (+) 594 WP_137362190.1 XTP/dITP diphosphatase -
  HV183_RS19935 (HV183_19940) hemW 4163488..4164624 (+) 1137 WP_181218119.1 radical SAM family heme chaperone HemW -
  HV183_RS19940 (HV183_19945) ansB 4164749..4165795 (-) 1047 WP_096755739.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35891.10 Da        Isoelectric Point: 6.4952

>NTDB_id=457733 HV183_RS19910 WP_181218114.1 4160320..4161300(-) (pilT) [Citrobacter freundii strain RHBSTW-00398]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTVPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPFDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
YRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQVGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=457733 HV183_RS19910 WP_181218114.1 4160320..4161300(-) (pilT) [Citrobacter freundii strain RHBSTW-00398]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGTGCCTGACGTGGAGAAGCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGTTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGATGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TACCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCACTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGTTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAGGTGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.082

97.546

0.42
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371