Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUK62_RS04860 Genome accession   NZ_CP055277
Coordinates   1045888..1046925 (-) Length   345 a.a.
NCBI ID   WP_004694862.1    Uniprot ID   N9CT59
Organism   Acinetobacter sp. NEB 394     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040888..1051925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK62_RS04830 (HUK62_04830) - 1041682..1042137 (+) 456 WP_176537230.1 bacteriohemerythrin -
  HUK62_RS04835 (HUK62_04835) - 1042280..1043362 (+) 1083 WP_176537231.1 hypothetical protein -
  HUK62_RS04840 (HUK62_04840) - 1043362..1043685 (+) 324 WP_176537232.1 RnfH family protein -
  HUK62_RS04845 (HUK62_04845) bamE 1043732..1044130 (-) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  HUK62_RS04850 (HUK62_04850) fur 1044243..1044680 (+) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  HUK62_RS04855 (HUK62_04855) pilU 1044740..1045861 (-) 1122 WP_010326019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUK62_RS04860 (HUK62_04860) pilT 1045888..1046925 (-) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  HUK62_RS04865 (HUK62_04865) - 1047048..1047737 (+) 690 WP_176537233.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUK62_RS04870 (HUK62_04870) - 1047928..1048662 (+) 735 WP_176537234.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38292.96 Da        Isoelectric Point: 6.6658

>NTDB_id=457377 HUK62_RS04860 WP_004694862.1 1045888..1046925(-) (pilT) [Acinetobacter sp. NEB 394]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPSIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQTFKELCELPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVTKGVISPQTARTAAKQPETFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=457377 HUK62_RS04860 WP_004694862.1 1045888..1046925(-) (pilT) [Acinetobacter sp. NEB 394]
ATGGATATCACAGAATTACTAGCCTTTTCGGCTAAGAACGGGGCATCAGATTTACACTTATCTGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATCAATTTACCAGCGTTAGAACACAAAGAAGTGCATAAACTGGTCTATGACA
TCATGAATGACAAACAACGTCGTGATTTCGAAGAAAAACTAGAAACAGACTTTTCCTTTGAAGTTCCAAGCATTGCTCGC
TTTCGTGTGAACGCATTCAACCAAAACCGCGGTGCAGGTGCAGTATTCCGTACCATTCCGTCTAAAGTCCTGACCATGGA
AGACTTAGGTTTAGGACAAACCTTCAAAGAGTTATGTGAATTGCCACGTGGTTTAGTTTTAGTGACGGGGCCTACCGGTT
CTGGTAAATCTACCACATTGGCGGCAATGATCGATTATATTAACGATAACCGCTATGACCATATCTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAGTCAAAAAAATGCTTGATCAACCAACGTGAAGTGCACCGTGATACACATGGTTTTAACGA
AGCACTGCGTTCAGCACTACGTGAAGACCCCGATATTATCCTCGTGGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGCTCACAGCAGCAGAAACGGGTCACTTGGTCTTCGGTACACTACATACCACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGTTATCTGAATCGCTACAAGCCGTGATTTCTCAAAC
CTTGCTCAAGAAAAATGGCGGTGGCCGTGTTGCCGCGCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAACAAAGTGGCACAGATGTATTCGGCTATTCAGACTGGTGCAAACTACGGTATGACCACACTGGATCAAAGTTTG
AAAAACTTGGTCACCAAAGGGGTGATCAGTCCACAGACTGCACGTACAGCTGCTAAGCAACCTGAAACCTTCTTATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.452

97.391

0.667
  pilT Vibrio cholerae strain A1552

68.452

97.391

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

42.183

98.261

0.414
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

39.942

99.42

0.397