Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HLB43_RS03875 Genome accession   NZ_CP055224
Coordinates   874380..875396 (+) Length   338 a.a.
NCBI ID   WP_011095128.1    Uniprot ID   Q6D122
Organism   Pectobacterium atrosepticum strain Green1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869380..880396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLB43_RS03835 (HLB43_03835) - 869425..869649 (-) 225 WP_080708297.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HLB43_RS03840 (HLB43_03840) - 869600..869860 (-) 261 WP_071598490.1 type II toxin-antitoxin system ParD family antitoxin -
  HLB43_RS03845 (HLB43_03845) hemW 870092..871231 (-) 1140 WP_011095133.1 radical SAM family heme chaperone HemW -
  HLB43_RS03850 (HLB43_03850) - 871224..871817 (-) 594 WP_011095132.1 XTP/dITP diphosphatase -
  HLB43_RS03855 (HLB43_03855) yggU 871835..872125 (-) 291 WP_005974847.1 DUF167 family protein YggU -
  HLB43_RS03860 (HLB43_03860) - 872122..872676 (-) 555 WP_011095131.1 YggT family protein -
  HLB43_RS03865 (HLB43_03865) proC 872726..873547 (-) 822 WP_011095130.1 pyrroline-5-carboxylate reductase -
  HLB43_RS03870 (HLB43_03870) - 873645..874358 (-) 714 WP_011095129.1 YggS family pyridoxal phosphate-dependent enzyme -
  HLB43_RS03875 (HLB43_03875) pilT 874380..875396 (+) 1017 WP_011095128.1 type IV pilus twitching motility protein PilT Machinery gene
  HLB43_RS03880 (HLB43_03880) - 875365..877197 (-) 1833 WP_011095127.1 diguanylate cyclase -
  HLB43_RS03885 (HLB43_03885) - 877728..879299 (-) 1572 WP_011095126.1 methyl-accepting chemotaxis protein -
  HLB43_RS03890 (HLB43_03890) nsrR 879596..880021 (+) 426 WP_011095125.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36718.01 Da        Isoelectric Point: 6.4221

>NTDB_id=456895 HLB43_RS03875 WP_011095128.1 874380..875396(+) (pilT) [Pectobacterium atrosepticum strain Green1]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPCLTPEQVAQWCDVWLEPTQQEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLDALHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMVGALNDSSDRHVITLE
DPIEFIHVSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLSTCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCVLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=456895 HLB43_RS03875 WP_011095128.1 874380..875396(+) (pilT) [Pectobacterium atrosepticum strain Green1]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGGCATCCTCCGGT
GTTGCGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCATGTTTAACGCCGGAGCAGGTGGCACAATGGTGCGATG
TCTGGCTAGAACCGACTCAGCAAGAGCAACTGCGGCAGGCAGGGCAGGTGGACGGCGCCTTAATGTTGCCTGACGGACAG
CGCCTGCGGGTAAATGTGTTTCGTCAGCGGGAAGGACTGTCTGCGGCGCTACGCATTATCCCATCTGTTCAACCTTCATT
GGATGCGCTACATGCGCCACCCGTGCTCTCGACGCTGCTGGAGAAACCAAACGGACTGATCCTGATTACCGGCGCGACGG
GCAGCGGTAAATCCACGACGCTAGCGGCGATGGTCGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATTCATGTCAGCCGACGTTGCCTGATTCAACAGCGAGAAATCGGTGCGCACAGCGCTTCTTTTGC
GCAGGCGCTACGGGCGGCATTGAGGGAAGATCCTGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACAGCAGCGGAAACTGGCCACTTGGTGTTATCAACGCTACATACGCGCAGTGCATCACAGGCAGTCGACCGT
TTGATTGATGTTTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTATCGACCTGTTTGCAGGCGGTAGTGACGCA
AAAGCTATTGCCCGCAGCGCAAGGCGGTCGAATTGCACTTTATGAAGTGCTGACTGCTACAGCGGCAGTCAGCAACCTGA
TTCGTGAGGGAAAAACGCACCAGCTTCCTGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGCCGGGATGGGCTTATTTCACACAGTTGCGTCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6D122

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Acinetobacter baylyi ADP1

48.225

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Pseudomonas aeruginosa PAK

48.64

97.929

0.476
  pilT Acinetobacter nosocomialis M2

48.338

97.929

0.473
  pilT Acinetobacter baumannii D1279779

48.338

97.929

0.473
  pilT Acinetobacter baumannii strain A118

48.338

97.929

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.342

98.521

0.417
  pilU Pseudomonas stutzeri DSM 10701

40.059

99.704

0.399
  pilU Vibrio cholerae strain A1552

38.872

99.704

0.388
  pilU Acinetobacter baylyi ADP1

38.82

95.266

0.37