Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUZ45_RS04195 Genome accession   NZ_CP055181
Coordinates   846922..847902 (+) Length   326 a.a.
NCBI ID   WP_123178612.1    Uniprot ID   -
Organism   Shigella flexneri strain STEFF_17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 841922..852902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUZ45_RS04165 (HUZ45_04125) - 842440..843447 (+) 1008 WP_128759177.1 DUF1202 family protein -
  HUZ45_RS04170 (HUZ45_04130) hemW 843599..844735 (-) 1137 WP_000239926.1 radical SAM family heme chaperone HemW -
  HUZ45_RS04175 (HUZ45_04135) - 844728..845321 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  HUZ45_RS04180 (HUZ45_04140) yggU 845329..845619 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  HUZ45_RS04185 (HUZ45_04145) yggT 845616..846182 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  HUZ45_RS04190 (HUZ45_04150) yggS 846200..846904 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  HUZ45_RS04195 (HUZ45_04155) pilT 846922..847902 (+) 981 WP_123178612.1 type IV pilus twitching motility protein PilT Machinery gene
  HUZ45_RS04200 (HUZ45_04160) ruvX 848086..848502 (-) 417 WP_000017109.1 Holliday junction resolvase RuvX -
  HUZ45_RS04205 (HUZ45_04165) - 848502..849065 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  HUZ45_RS04210 (HUZ45_04170) gshB 849174..850124 (-) 951 WP_000593273.1 glutathione synthase -
  HUZ45_RS04215 (HUZ45_04175) rsmE 850137..850868 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HUZ45_RS04220 (HUZ45_04180) endA 850948..851655 (-) 708 WP_001613793.1 deoxyribonuclease I -
  HUZ45_RS04225 (HUZ45_04185) - 851750..852247 (-) 498 WP_001370180.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35897.13 Da        Isoelectric Point: 5.3988

>NTDB_id=456690 HUZ45_RS04195 WP_123178612.1 846922..847902(+) (pilT) [Shigella flexneri strain STEFF_17]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSCCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLVLTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=456690 HUZ45_RS04195 WP_123178612.1 846922..847902(+) (pilT) [Shigella flexneri strain STEFF_17]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGTTGTTACCTTCGTGCTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGTAAAT
CCACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGTACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365