Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUW55_RS04170 Genome accession   NZ_CP055161
Coordinates   892397..893419 (+) Length   340 a.a.
NCBI ID   WP_049300548.1    Uniprot ID   -
Organism   Serratia marcescens strain JW-CZ2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 887397..898419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW55_RS04135 - 887798..888046 (+) 249 WP_151254410.1 DUF2442 domain-containing protein -
  HUW55_RS04140 hemW 888083..889225 (-) 1143 WP_176062668.1 radical SAM family heme chaperone HemW -
  HUW55_RS04145 - 889218..889811 (-) 594 WP_049300550.1 XTP/dITP diphosphatase -
  HUW55_RS04150 yggU 889952..890245 (-) 294 WP_143779451.1 DUF167 family protein YggU -
  HUW55_RS04155 - 890242..890796 (-) 555 WP_016930090.1 YggT family protein -
  HUW55_RS04160 proC 890828..891649 (-) 822 WP_033639391.1 pyrroline-5-carboxylate reductase -
  HUW55_RS04165 - 891669..892376 (-) 708 WP_049300549.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUW55_RS04170 pilT 892397..893419 (+) 1023 WP_049300548.1 type IV pilus twitching motility protein PilT Machinery gene
  HUW55_RS04175 ruvX 893416..893838 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  HUW55_RS04180 - 893838..894401 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  HUW55_RS04185 gshB 894548..895498 (-) 951 WP_176062670.1 glutathione synthase -
  HUW55_RS04190 rsmE 895509..896240 (-) 732 WP_049300547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HUW55_RS04195 endA 896310..897005 (-) 696 WP_176062672.1 deoxyribonuclease I -
  HUW55_RS04200 - 897107..897619 (-) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36405.64 Da        Isoelectric Point: 7.0670

>NTDB_id=456561 HUW55_RS04170 WP_049300548.1 892397..893419(+) (pilT) [Serratia marcescens strain JW-CZ2]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAAQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=456561 HUW55_RS04170 WP_049300548.1 892397..893419(+) (pilT) [Serratia marcescens strain JW-CZ2]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGCGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATTGTCCCGGCGTTGCTGCGGCGCGACGACGGGCTTATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGTTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCCGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.665

99.118

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Vibrio cholerae strain A1552

39.815

95.294

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368