Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JGU77_RS04680 Genome accession   NZ_CP066806
Coordinates   964643..965623 (+) Length   326 a.a.
NCBI ID   WP_075862143.1    Uniprot ID   -
Organism   Escherichia coli strain TCM3e1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 959643..970623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGU77_RS04650 (JGU77_04650) - 960021..961007 (-) 987 WP_001564022.1 TRAP transporter substrate-binding protein -
  JGU77_RS04655 (JGU77_04655) hemW 961320..962456 (-) 1137 WP_075862142.1 radical SAM family heme chaperone HemW -
  JGU77_RS04660 (JGU77_04660) rdgB 962449..963042 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  JGU77_RS04665 (JGU77_04665) yggU 963050..963340 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  JGU77_RS04670 (JGU77_04670) yggT 963337..963903 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  JGU77_RS04675 (JGU77_04675) yggS 963921..964625 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  JGU77_RS04680 (JGU77_04680) pilT 964643..965623 (+) 981 WP_075862143.1 type IV pilus twitching motility protein PilT Machinery gene
  JGU77_RS04685 (JGU77_04685) ruvX 965836..966252 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  JGU77_RS04690 (JGU77_04690) yqgE 966252..966815 (-) 564 WP_001339287.1 YqgE/AlgH family protein -
  JGU77_RS04695 (JGU77_04695) gshB 966924..967874 (-) 951 WP_075862144.1 glutathione synthase -
  JGU77_RS04700 (JGU77_04700) rsmE 967887..968618 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JGU77_RS04705 (JGU77_04705) endA 968698..969405 (-) 708 WP_000286500.1 deoxyribonuclease I -
  JGU77_RS04710 (JGU77_04710) yggI 969500..969997 (-) 498 WP_023908971.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35929.12 Da        Isoelectric Point: 5.5860

>NTDB_id=456422 JGU77_RS04680 WP_075862143.1 964643..965623(+) (pilT) [Escherichia coli strain TCM3e1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPIE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=456422 JGU77_RS04680 WP_075862143.1 964643..965623(+) (pilT) [Escherichia coli strain TCM3e1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAAAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTATTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTAGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCAGTTTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCAGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371