Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUT23_RS29585 Genome accession   NZ_CP054871
Coordinates   6520761..6521795 (-) Length   344 a.a.
NCBI ID   WP_210483031.1    Uniprot ID   -
Organism   Pseudomonas protegens strain PPRAR07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6515761..6526795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUT23_RS29555 (HUT23_29555) ruvX 6515904..6516341 (+) 438 WP_011064005.1 Holliday junction resolvase RuvX -
  HUT23_RS29560 (HUT23_29560) pyrR 6516457..6516963 (+) 507 WP_011064006.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  HUT23_RS29565 (HUT23_29565) - 6516989..6517993 (+) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  HUT23_RS29570 (HUT23_29570) - 6517990..6519261 (+) 1272 WP_011064008.1 dihydroorotase -
  HUT23_RS29575 (HUT23_29575) - 6519389..6519808 (-) 420 WP_011064009.1 TM2 domain-containing protein -
  HUT23_RS29580 (HUT23_29580) - 6520059..6520682 (+) 624 WP_011064010.1 C40 family peptidase -
  HUT23_RS29585 (HUT23_29585) pilT 6520761..6521795 (-) 1035 WP_210483031.1 type IV pilus twitching motility protein PilT Machinery gene
  HUT23_RS29590 (HUT23_29590) - 6521986..6522684 (+) 699 WP_041120251.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUT23_RS29595 (HUT23_29595) proC 6522719..6523537 (+) 819 WP_011064013.1 pyrroline-5-carboxylate reductase -
  HUT23_RS29600 (HUT23_29600) - 6523548..6524138 (+) 591 WP_011064014.1 YggT family protein -
  HUT23_RS29605 (HUT23_29605) - 6524153..6524446 (+) 294 WP_011064015.1 DUF167 domain-containing protein -
  HUT23_RS29610 (HUT23_29610) - 6524610..6525749 (+) 1140 WP_041752663.1 homoserine O-acetyltransferase -
  HUT23_RS29615 (HUT23_29615) metW 6525757..6526377 (+) 621 WP_011064017.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37426.15 Da        Isoelectric Point: 6.8353

>NTDB_id=455123 HUT23_RS29585 WP_210483031.1 6520761..6521795(-) (pilT) [Pseudomonas protegens strain PPRAR07]
MDITELLRLSASRGASDLHLSAGLAPMLRIDGDIRPLPGPALEGPQVQALIHELMSEAQRQAFAACNDLDFAYELPGVAR
FRVNAFRHARGVGAVLRLIPARVQSLEELGLGEVFRQITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNQHRQQHILTIED
PIEFVHPQKNCLVHQREVQRDTQSFSTALRAALREDPDVILLGELRDLETIRLALTAAETGHLVLATLHTSSAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVVAQVLVKKVGGGRVAAHEIMLGSPAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLAQGVISRDQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=455123 HUT23_RS29585 WP_210483031.1 6520761..6521795(-) (pilT) [Pseudomonas protegens strain PPRAR07]
ATGGACATCACCGAACTCTTGCGCTTGAGCGCTTCCCGGGGCGCTTCGGACCTGCATCTGTCCGCCGGGCTGGCGCCGAT
GTTGCGCATCGATGGCGATATTCGCCCGCTGCCGGGCCCGGCCCTGGAAGGGCCACAGGTGCAGGCGCTGATCCATGAAC
TGATGAGCGAAGCACAGCGCCAGGCGTTCGCCGCCTGCAATGACCTGGATTTCGCCTATGAACTGCCGGGTGTGGCGCGG
TTTCGGGTCAATGCCTTCCGGCACGCCCGGGGCGTCGGTGCGGTGCTGCGCCTGATTCCCGCGCGGGTACAGAGCCTGGA
GGAGCTGGGACTGGGGGAAGTGTTTCGGCAGATTACCGAGGCGCCCCGCGGGCTGGTGCTGGTGACCGGGCCCACCGGCA
GCGGCAAGTCCACCACACTGGCGGCGATGATCGACTACCTGAACCAGCACCGCCAGCAGCACATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCATCCGCAGAAGAACTGCCTGGTGCACCAGCGCGAAGTGCAGCGCGATACCCAGAGCTTCTCCAC
GGCCTTGCGTGCGGCGCTGCGGGAAGATCCCGACGTGATCCTGCTGGGCGAACTGCGGGACCTGGAAACCATCCGCCTGG
CCCTGACCGCCGCGGAAACCGGGCACCTGGTACTGGCCACCTTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGCTG
GTGGACGTGTTTCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGCTGCAGGCAGTGGTCGCGCAAGT
GCTGGTGAAGAAGGTGGGCGGTGGCCGAGTAGCGGCGCACGAGATCATGCTGGGCAGCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACGGGCGGCGCCCAGGGCATGCGCACCCTGGACATGAGCCTC
AAGGCACTGCTCGCTCAAGGGGTGATCAGCCGCGATCAGGCGCGGGAGCAGGCGCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.512

100

0.715
  pilT Pseudomonas stutzeri DSM 10701

71.512

100

0.715
  pilT Acinetobacter baumannii D1279779

66.86

100

0.669
  pilT Acinetobacter baumannii strain A118

66.86

100

0.669
  pilT Acinetobacter nosocomialis M2

66.57

100

0.666
  pilT Acinetobacter baylyi ADP1

66.57

100

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.529

98.837

0.628
  pilT Vibrio cholerae strain A1552

63.529

98.837

0.628
  pilT Legionella pneumophila strain Lp02

62.13

98.256

0.61
  pilT Legionella pneumophila strain ERS1305867

62.13

98.256

0.61
  pilT Neisseria meningitidis 8013

57.971

100

0.581
  pilT Neisseria gonorrhoeae MS11

57.681

100

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

42.687

97.384

0.416
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387