Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUT27_RS27805 Genome accession   NZ_CP054867
Coordinates   6207618..6208652 (-) Length   344 a.a.
NCBI ID   WP_009051312.1    Uniprot ID   A0AB33WT94
Organism   Pseudomonas chlororaphis strain PCLRT02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6202618..6213652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUT27_RS27775 (HUT27_27775) ruvX 6202763..6203200 (+) 438 WP_009046019.1 Holliday junction resolvase RuvX -
  HUT27_RS27780 (HUT27_27780) pyrR 6203322..6203828 (+) 507 WP_007929976.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  HUT27_RS27785 (HUT27_27785) - 6203855..6204859 (+) 1005 WP_007929978.1 aspartate carbamoyltransferase catalytic subunit -
  HUT27_RS27790 (HUT27_27790) - 6204856..6206127 (+) 1272 WP_124315464.1 dihydroorotase -
  HUT27_RS27795 (HUT27_27795) - 6206233..6206652 (-) 420 WP_009051311.1 TM2 domain-containing protein -
  HUT27_RS27800 (HUT27_27800) - 6206903..6207535 (+) 633 WP_009046022.1 C40 family peptidase -
  HUT27_RS27805 (HUT27_27805) pilT 6207618..6208652 (-) 1035 WP_009051312.1 type IV pilus twitching motility protein PilT Machinery gene
  HUT27_RS27810 (HUT27_27810) - 6208710..6209408 (+) 699 WP_009051313.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUT27_RS27815 (HUT27_27815) proC 6209443..6210261 (+) 819 WP_210532657.1 pyrroline-5-carboxylate reductase -
  HUT27_RS27820 (HUT27_27820) - 6210272..6210862 (+) 591 WP_009051315.1 YggT family protein -
  HUT27_RS27825 (HUT27_27825) - 6210876..6211169 (+) 294 WP_028683556.1 DUF167 domain-containing protein -
  HUT27_RS27830 (HUT27_27830) - 6211343..6212482 (+) 1140 WP_009051317.1 homoserine O-acetyltransferase -
  HUT27_RS27835 (HUT27_27835) metW 6212490..6213110 (+) 621 WP_007921185.1 methionine biosynthesis protein MetW -
  HUT27_RS27840 (HUT27_27840) - 6213138..6213572 (+) 435 WP_028683555.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37450.14 Da        Isoelectric Point: 7.1604

>NTDB_id=455073 HUT27_RS27805 WP_009051312.1 6207618..6208652(-) (pilT) [Pseudomonas chlororaphis strain PCLRT02]
MDITELLAFSVKQGASDLHLSAGLPPMIRIDGDIRRINLPVLEAQQVHELILASMTDPQRAEFKAALEIDFSFAVPGLAR
FRVNAFNHQRGAGAVFRSIPAKVPSLDELGMGEVFRRISEVPRGLVLVTGPTGSGKSTTLAAMIDHLNQSRHQHILTIED
PIEFIHESKKCLINQREVHRDTHSFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRV
VDVFPAGEKAMVRSMLSESLQAVISQALVKRIGGGRVAAHEIMLGSAAIRNLIREDKVAQMYSAIQTGGAQGMQTLDMSL
KALVDAGLVSREHAREKARAPESI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=455073 HUT27_RS27805 WP_009051312.1 6207618..6208652(-) (pilT) [Pseudomonas chlororaphis strain PCLRT02]
ATGGATATCACTGAGTTGCTGGCCTTCAGTGTCAAACAGGGCGCTTCGGACCTGCACCTCTCGGCCGGTTTGCCGCCGAT
GATTCGCATCGACGGCGATATTCGCCGGATCAACCTGCCGGTGCTGGAGGCGCAACAGGTGCACGAGCTGATCCTGGCCA
GCATGACGGACCCGCAGCGGGCCGAGTTCAAGGCCGCGCTGGAGATCGACTTTTCCTTCGCGGTGCCGGGCCTGGCGCGC
TTTCGGGTCAATGCCTTCAACCATCAGCGCGGCGCTGGCGCGGTCTTTCGCAGCATTCCGGCCAAGGTGCCGAGCCTGGA
CGAACTGGGCATGGGCGAGGTGTTTCGCCGCATCAGCGAAGTGCCCCGCGGCCTGGTACTGGTGACCGGACCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGATCGATCACCTGAACCAGAGCCGGCACCAGCACATCCTCACCATCGAGGAC
CCCATCGAGTTCATCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCACAGCTTCTCCAC
GGCGCTGCGTTCGGCCCTGCGCGAGGACCCGGATGTGATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CGTTGACCGCCGCGGAGACCGGGCATCTGGTGTTCGGTACCCTGCACACCACCTCGGCGGCGAAAACCGTGGACCGGGTG
GTGGATGTGTTTCCGGCCGGGGAGAAGGCGATGGTCCGCTCCATGCTCTCTGAATCGCTGCAGGCGGTGATTTCCCAGGC
GCTGGTGAAGCGGATCGGCGGAGGCCGGGTAGCGGCCCACGAGATCATGCTGGGCAGCGCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGGGCGCAGGGGATGCAGACCCTGGACATGAGCCTG
AAGGCGCTGGTGGATGCCGGGCTGGTCAGCCGGGAGCACGCCCGGGAGAAGGCGCGGGCGCCGGAGAGTATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.105

100

0.811
  pilT Pseudomonas stutzeri DSM 10701

78.198

100

0.782
  pilT Acinetobacter nosocomialis M2

74.128

100

0.741
  pilT Acinetobacter baumannii D1279779

74.128

100

0.741
  pilT Acinetobacter baumannii strain A118

74.128

100

0.741
  pilT Acinetobacter baylyi ADP1

72.674

100

0.727
  pilT Legionella pneumophila strain Lp02

67.751

98.256

0.666
  pilT Legionella pneumophila strain ERS1305867

67.751

98.256

0.666
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.965

0.651
  pilT Vibrio cholerae strain A1552

66.469

97.965

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.02

99.709

0.509
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

40.896

97.384

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387