Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HUO05_RS10505 Genome accession   NZ_CP054700
Coordinates   2262397..2263503 (-) Length   368 a.a.
NCBI ID   WP_176311163.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain GS_MYPK1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2257397..2268503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUO05_RS10475 (HUO05_10475) - 2258030..2258527 (+) 498 WP_005381025.1 SprT family zinc-dependent metalloprotease -
  HUO05_RS10480 (HUO05_10480) endA 2258678..2259373 (+) 696 WP_005381023.1 deoxyribonuclease I -
  HUO05_RS10485 (HUO05_10485) rsmE 2259487..2260218 (+) 732 WP_005381022.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HUO05_RS10490 (HUO05_10490) gshB 2260232..2261182 (+) 951 WP_005381021.1 glutathione synthase -
  HUO05_RS10495 (HUO05_10495) - 2261311..2261874 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  HUO05_RS10500 (HUO05_10500) ruvX 2261910..2262335 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  HUO05_RS10505 (HUO05_10505) pilU 2262397..2263503 (-) 1107 WP_176311163.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUO05_RS10510 (HUO05_10510) pilT 2263534..2264574 (-) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  HUO05_RS10515 (HUO05_10515) - 2264603..2265304 (+) 702 WP_176311164.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUO05_RS10520 (HUO05_10520) proC 2265464..2266282 (+) 819 WP_042521294.1 pyrroline-5-carboxylate reductase -
  HUO05_RS10525 (HUO05_10525) - 2266335..2266892 (+) 558 WP_005381012.1 YggT family protein -
  HUO05_RS10530 (HUO05_10530) yggU 2266892..2267182 (+) 291 WP_005381011.1 DUF167 family protein YggU -
  HUO05_RS10535 (HUO05_10535) - 2267311..2267742 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  HUO05_RS10540 (HUO05_10540) - 2267861..2268463 (+) 603 WP_005381009.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41283.40 Da        Isoelectric Point: 6.7734

>NTDB_id=454025 HUO05_RS10505 WP_176311163.1 2262397..2263503(-) (pilU) [Vibrio alginolyticus strain GS_MYPK1]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=454025 HUO05_RS10505 WP_176311163.1 2262397..2263503(-) (pilU) [Vibrio alginolyticus strain GS_MYPK1]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTTACCGAGAGCGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGATTGTGGTCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACCAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGATCGGGTA
AATCGACCACCATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAATTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGCGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGCTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGCGAATGATCTGCGCTTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

92.12

0.405
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361