Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FOC72_RS10650 Genome accession   NZ_CP054570
Coordinates   2183217..2184281 (+) Length   354 a.a.
NCBI ID   WP_002894192.1    Uniprot ID   A0A3R9I5P7
Organism   Streptococcus sanguinis strain FDAARGOS_770     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2178217..2189281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOC72_RS10635 (FOC72_10635) - 2178832..2179353 (-) 522 WP_002894195.1 TetR/AcrR family transcriptional regulator -
  FOC72_RS10640 (FOC72_10640) - 2179523..2180410 (+) 888 WP_002894194.1 cation diffusion facilitator family transporter -
  FOC72_RS10645 (FOC72_10645) - 2181533..2183215 (+) 1683 WP_002894193.1 GspE/PulE family protein -
  FOC72_RS10650 (FOC72_10650) pilT 2183217..2184281 (+) 1065 WP_002894192.1 type IV pilus twitching motility protein PilT Machinery gene
  FOC72_RS10655 (FOC72_10655) - 2184285..2185511 (+) 1227 WP_002894191.1 type II secretion system F family protein -
  FOC72_RS10660 (FOC72_10660) - 2185681..2186142 (+) 462 WP_032914015.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FOC72_RS10665 (FOC72_10665) - 2186284..2186742 (+) 459 WP_002894189.1 type II secretion system protein -
  FOC72_RS10670 (FOC72_10670) - 2186815..2187306 (+) 492 WP_002894187.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FOC72_RS10675 (FOC72_10675) - 2187439..2187933 (+) 495 WP_002894185.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39271.34 Da        Isoelectric Point: 7.0215

>NTDB_id=453530 FOC72_RS10650 WP_002894192.1 2183217..2184281(+) (pilT) [Streptococcus sanguinis strain FDAARGOS_770]
MNLDELIKQAIEAGASDIHFTVGAEPTMRLHGRLTQLNNTILTNEDTVRFTKHILNDKQIAHLLEVGEVDCAYEVDNGYR
LRVNIFKQKNNCGIALRVIPKDIPSMESLGLPPVIKKLAEKRRGLILVTGPTGSGKSTTLATMINYMNSLRDEHIITIED
PIEYMHTHNKCLVNQRELGADTQSFGNALRGALRQDPDVILVGEMRDKETIEIALRAAETGHLVLSTLHTVGAVNTMDRI
IDVFPAEQQEQIRVQLSAVMEGVVSQQLMRTATGKGRVAAFEIMLGTPAIRNLIREGKTHQLLTPIQTGAQLGMITMDTS
LMGLYRRNVIDQRTLLSYSVDRAQVEKSLGIVGY

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=453530 FOC72_RS10650 WP_002894192.1 2183217..2184281(+) (pilT) [Streptococcus sanguinis strain FDAARGOS_770]
ATGAATTTGGATGAGTTAATTAAGCAGGCGATTGAAGCGGGTGCATCAGATATCCACTTCACGGTTGGCGCTGAGCCAAC
CATGCGTCTGCACGGGAGGTTAACACAACTCAACAATACAATTTTGACCAACGAAGACACGGTTCGCTTCACCAAGCACA
TTTTAAATGATAAGCAGATTGCTCATCTTTTAGAAGTCGGTGAAGTGGACTGTGCCTACGAGGTCGACAATGGCTATCGC
TTGCGGGTCAATATCTTTAAGCAGAAAAACAACTGCGGGATTGCCTTGCGGGTCATTCCCAAGGATATTCCATCTATGGA
ATCTCTGGGATTGCCACCAGTTATCAAAAAGCTGGCTGAGAAACGCCGGGGGCTGATATTGGTTACCGGGCCAACAGGAA
GCGGAAAGTCAACGACCCTGGCAACCATGATCAACTACATGAACAGCTTGCGGGACGAGCACATCATCACGATTGAAGAT
CCGATCGAGTACATGCATACGCATAATAAGTGCTTGGTCAACCAGCGTGAGCTGGGAGCGGATACGCAGAGCTTCGGCAA
TGCTCTCCGCGGTGCCCTTCGTCAGGACCCGGATGTCATCCTAGTCGGTGAGATGCGGGATAAGGAAACGATTGAAATCG
CACTGCGTGCGGCGGAAACAGGTCACTTAGTTCTGTCAACTCTCCACACGGTCGGTGCCGTAAACACCATGGACCGGATT
ATCGACGTATTTCCAGCCGAGCAGCAGGAGCAAATCCGCGTACAGCTTTCAGCCGTTATGGAAGGGGTTGTATCGCAGCA
GCTTATGCGTACAGCGACCGGCAAAGGTCGTGTAGCTGCCTTTGAAATCATGCTGGGAACACCCGCTATTCGCAACCTCA
TTCGCGAAGGGAAGACCCACCAGCTGCTGACGCCAATCCAAACAGGGGCTCAGCTAGGCATGATTACCATGGACACTTCG
CTCATGGGACTCTACCGTCGCAATGTTATCGACCAAAGAACTCTGCTGTCCTACTCAGTAGACCGCGCTCAAGTTGAAAA
ATCCCTAGGAATAGTGGGGTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R9I5P7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

51.796

94.35

0.489
  pilT Acinetobacter nosocomialis M2

51.796

94.35

0.489
  pilT Acinetobacter baumannii D1279779

51.796

94.35

0.489
  pilT Legionella pneumophila strain Lp02

51.198

94.35

0.483
  pilT Legionella pneumophila strain ERS1305867

51.198

94.35

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.742

95.198

0.483
  pilT Vibrio cholerae strain A1552

50.742

95.198

0.483
  pilT Pseudomonas aeruginosa PAK

50.602

93.785

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.974

96.328

0.472
  pilT Pseudomonas stutzeri DSM 10701

50.301

93.785

0.472
  pilT Acinetobacter baylyi ADP1

50.151

93.503

0.469
  pilT Neisseria meningitidis 8013

49.697

93.22

0.463
  pilT Neisseria gonorrhoeae MS11

49.394

93.22

0.46
  pilU Pseudomonas stutzeri DSM 10701

44.308

91.808

0.407
  pilU Vibrio cholerae strain A1552

41.399

96.893

0.401
  pilU Acinetobacter baylyi ADP1

41.159

92.655

0.381