Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HS042_RS10805 Genome accession   NZ_CP054277
Coordinates   2280344..2281366 (-) Length   340 a.a.
NCBI ID   WP_075684624.1    Uniprot ID   -
Organism   Serratia marcescens strain Byron     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2275344..2286366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS042_RS10775 (HS042_10775) - 2276138..2276650 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  HS042_RS10780 (HS042_10780) endA 2276752..2277447 (+) 696 WP_160125768.1 deoxyribonuclease I -
  HS042_RS10785 (HS042_10785) rsmE 2277517..2278248 (+) 732 WP_015379044.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HS042_RS10790 (HS042_10790) gshB 2278259..2279209 (+) 951 WP_016930084.1 glutathione synthase -
  HS042_RS10795 (HS042_10795) - 2279362..2279925 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  HS042_RS10800 (HS042_10800) ruvX 2279925..2280347 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  HS042_RS10805 (HS042_10805) pilT 2280344..2281366 (-) 1023 WP_075684624.1 type IV pilus twitching motility protein PilT Machinery gene
  HS042_RS10810 (HS042_10810) - 2281387..2282094 (+) 708 WP_015379048.1 YggS family pyridoxal phosphate-dependent enzyme -
  HS042_RS10815 (HS042_10815) proC 2282114..2282935 (+) 822 WP_160125769.1 pyrroline-5-carboxylate reductase -
  HS042_RS10820 (HS042_10820) - 2282967..2283521 (+) 555 WP_004937436.1 YggT family protein -
  HS042_RS10825 (HS042_10825) yggU 2283518..2283808 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  HS042_RS10830 (HS042_10830) - 2283827..2284420 (+) 594 WP_049239920.1 XTP/dITP diphosphatase -
  HS042_RS10835 (HS042_10835) hemW 2284413..2285555 (+) 1143 WP_160125770.1 radical SAM family heme chaperone HemW -
  HS042_RS10840 (HS042_10840) - 2285591..2285839 (-) 249 WP_004937422.1 DUF2442 domain-containing protein -
  HS042_RS10845 (HS042_10845) - 2285823..2286065 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36359.66 Da        Isoelectric Point: 7.9374

>NTDB_id=451767 HS042_RS10805 WP_075684624.1 2280344..2281366(-) (pilT) [Serratia marcescens strain Byron]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAALAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTRG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=451767 HS042_RS10805 WP_075684624.1 2280344..2281366(-) (pilT) [Serratia marcescens strain Byron]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAAGCGCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACCGGCCGAGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAGTCGCCTTCGCT
TGCCGCGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACTTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGATGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAGGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAGGGAACGAGGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Pseudomonas aeruginosa PAK

48.788

97.059

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.258

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365