Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HR071_RS08630 Genome accession   NZ_CP054232
Coordinates   1799650..1800630 (-) Length   326 a.a.
NCBI ID   WP_001525611.1    Uniprot ID   A0A6D0HCQ9
Organism   Escherichia coli strain EcPF7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1794650..1805630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR071_RS08600 (HR071_08595) yggI 1795315..1795812 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  HR071_RS08605 (HR071_08600) endA 1795907..1796614 (+) 708 WP_001305312.1 deoxyribonuclease I -
  HR071_RS08610 (HR071_08605) rsmE 1796694..1797425 (+) 732 WP_001531929.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HR071_RS08615 (HR071_08610) gshB 1797438..1798388 (+) 951 WP_000593260.1 glutathione synthase -
  HR071_RS08620 (HR071_08615) yqgE 1798497..1799060 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  HR071_RS08625 (HR071_08620) ruvX 1799060..1799476 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  HR071_RS08630 (HR071_08625) pilT 1799650..1800630 (-) 981 WP_001525611.1 type IV pilus twitching motility protein PilT Machinery gene
  HR071_RS08635 (HR071_08630) yggS 1800648..1801352 (+) 705 WP_000997805.1 pyridoxal phosphate homeostasis protein -
  HR071_RS08640 (HR071_08635) yggT 1801370..1801936 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  HR071_RS08645 (HR071_08640) yggU 1801933..1802223 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  HR071_RS08650 (HR071_08645) rdgB 1802231..1802824 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  HR071_RS08655 (HR071_08650) hemW 1802817..1803953 (+) 1137 WP_000239974.1 radical SAM family heme chaperone HemW -
  HR071_RS08660 (HR071_08655) yggM 1804022..1805029 (-) 1008 WP_000745192.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35986.18 Da        Isoelectric Point: 5.7995

>NTDB_id=451595 HR071_RS08630 WP_001525611.1 1799650..1800630(-) (pilT) [Escherichia coli strain EcPF7]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFEAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=451595 HR071_RS08630 WP_001525611.1 1799650..1800630(-) (pilT) [Escherichia coli strain EcPF7]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGAGGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGAGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6D0HCQ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362