Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HQ912_RS26595 Genome accession   NZ_CP054128
Coordinates   5993584..5994618 (-) Length   344 a.a.
NCBI ID   WP_107321399.1    Uniprot ID   -
Organism   Pseudomonas sp. MPDS     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5988584..5999618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ912_RS26565 (HQ912_26565) ruvX 5988702..5989139 (+) 438 WP_047226001.1 Holliday junction resolvase RuvX -
  HQ912_RS26570 (HQ912_26570) pyrR 5989225..5989731 (+) 507 WP_047226002.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  HQ912_RS26575 (HQ912_26575) - 5989756..5990760 (+) 1005 WP_047226003.1 aspartate carbamoyltransferase catalytic subunit -
  HQ912_RS26580 (HQ912_26580) - 5990757..5992028 (+) 1272 WP_076383024.1 dihydroorotase -
  HQ912_RS26585 (HQ912_26585) - 5992150..5992578 (-) 429 WP_047226005.1 NINE protein -
  HQ912_RS26590 (HQ912_26590) - 5992828..5993439 (+) 612 WP_173407475.1 C40 family peptidase -
  HQ912_RS26595 (HQ912_26595) pilT 5993584..5994618 (-) 1035 WP_107321399.1 type IV pilus twitching motility protein PilT Machinery gene
  HQ912_RS26600 (HQ912_26600) - 5994673..5995359 (+) 687 WP_047226008.1 YggS family pyridoxal phosphate-dependent enzyme -
  HQ912_RS26605 (HQ912_26605) proC 5995402..5996220 (+) 819 WP_047226009.1 pyrroline-5-carboxylate reductase -
  HQ912_RS26610 (HQ912_26610) - 5996231..5996821 (+) 591 WP_047226010.1 YggT family protein -
  HQ912_RS26615 (HQ912_26615) - 5997063..5998202 (+) 1140 WP_047226011.1 homoserine O-acetyltransferase -
  HQ912_RS26620 (HQ912_26620) metW 5998210..5998830 (+) 621 WP_047226012.1 methionine biosynthesis protein MetW -
  HQ912_RS26625 (HQ912_26625) - 5998856..5999290 (+) 435 WP_047226013.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38178.80 Da        Isoelectric Point: 6.9776

>NTDB_id=450937 HQ912_RS26595 WP_107321399.1 5993584..5994618(-) (pilT) [Pseudomonas sp. MPDS]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQSFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLLNKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=450937 HQ912_RS26595 WP_107321399.1 5993584..5994618(-) (pilT) [Pseudomonas sp. MPDS]
ATGGATATCACTGAGCTACTGGCGTTCAGCGCCAAACAGGGCGCATCGGACCTGCACTTGTCCGCCGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTGAGGCGGATCAACCTGCCGGCCCTGGACCACAAGCAGGTCCATGAGCTGATCTACGACA
TCATGAACGACCGGCAGCGGGTAGATTACGAGAAGTTTCTGGAAACCGACTTTTCCTTCGACGTGCCGGGCGTCGCACGG
TTCCGGGTCAATGCGTTCAACCAGAATCGCGGTGCAGGTGCGGTGTTCCGGACCATCCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGGATGGGCGATGTGTTTCGCAAGGTGACCGAGGCGCCTCGCGGGCTGGTGTTGGTGACCGGGCCGACCGGCT
CCGGCAAGTCCACCACCCTGGCGGCGATGATCGACTACCTCAACACCCACAAACACCATCACATCCTCACCATCGAAGAT
CCCATCGAGTTTGTCCACGAGCCGCGCAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCAAAGTTTCTCCAC
GGCCCTGCGCTCAGCCCTGCGCGAAGACCCGGACGTGATTCTGGTGGGGGAGATGCGTGACCTGGAGACCATCCGCCTTG
CCCTGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCTTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCGGGGGATGAGAAATCCATGGTTCGTTCGATGTTGTCCGAATCCTTGCAGGCGGTGATTTCACAGAC
GCTGGTCAAGAAGATTGGCGGCGGCCGGGTCGCGGCTCATGAAATCATGCTGGGAACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGTGGGTCGCTGGGGATGCAGACATTGGATATGTGTTTG
AAGGATTTGCTGAACAAGGGGCTGATCAGTCGCGAACATGCGCGGGAGCGGGCGCGTACGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.42

100

0.395