Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A8O29_RS04280 Genome accession   NZ_CP054058
Coordinates   729571..730551 (+) Length   326 a.a.
NCBI ID   WP_125352862.1    Uniprot ID   -
Organism   Scandinavium goeteborgense strain CCUG 66741     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 724571..735551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8O29_RS04245 (A8O29_004245) - 724906..725625 (+) 720 WP_125352872.1 DUF2884 domain-containing protein -
  A8O29_RS04250 (A8O29_004250) - 725823..726173 (-) 351 WP_125352870.1 endonuclease domain-containing protein -
  A8O29_RS04255 (A8O29_004255) hemW 726237..727379 (-) 1143 WP_125352868.1 radical SAM family heme chaperone HemW -
  A8O29_RS04260 (A8O29_004260) - 727379..727966 (-) 588 WP_125352866.1 XTP/dITP diphosphatase -
  A8O29_RS04265 (A8O29_004265) yggU 727978..728265 (-) 288 WP_125352864.1 DUF167 family protein YggU -
  A8O29_RS04270 (A8O29_004270) - 728262..728828 (-) 567 WP_110511938.1 YggT family protein -
  A8O29_RS04275 (A8O29_004275) - 728851..729555 (-) 705 WP_125352882.1 YggS family pyridoxal phosphate-dependent enzyme -
  A8O29_RS04280 (A8O29_004280) pilT 729571..730551 (+) 981 WP_125352862.1 type IV pilus twitching motility protein PilT Machinery gene
  A8O29_RS04285 (A8O29_004285) - 730548..732806 (-) 2259 WP_174081220.1 FAD-dependent oxidoreductase -
  A8O29_RS04290 (A8O29_004290) - 732874..733566 (+) 693 WP_125352860.1 IclR family transcriptional regulator domain-containing protein -
  A8O29_RS04295 (A8O29_004295) - 734091..734744 (+) 654 WP_125352858.1 LuxR C-terminal-related transcriptional regulator -
  A8O29_RS04300 (A8O29_004300) ruvX 734809..735225 (-) 417 WP_110511944.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35649.70 Da        Isoelectric Point: 6.3089

>NTDB_id=450466 A8O29_RS04280 WP_125352862.1 729571..730551(+) (pilT) [Scandinavium goeteborgense strain CCUG 66741]
MDMQEIVALSVKHNVSDLHLCGDGTVRWRRRGHLEPAPFSAPEPQALLQNWLSETAMRQWEKDNQCDFAVQTDSGTRLRA
SAFRHADGVSIVLRLLPEICPQLEELGVPQAINELLAGESGLILVTGATGSGKSTTLAAMVDYLNHQRDGHILTLEDPVE
FVHHSERCLIHQREVGEHCGSFSAALRVALRQDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRGAAQAIERLVDV
FPAGEKEQVRSQLSGSLCAVLAQKLLPDKQGGRVALYELLVNTPAVGSLIRDGKTHQLPGVMQAGQQMGMMTFSQCFQQR
QAAGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=450466 A8O29_RS04280 WP_125352862.1 729571..730551(+) (pilT) [Scandinavium goeteborgense strain CCUG 66741]
ATGGATATGCAGGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCGGCGATGGCACGGTGCG
CTGGCGGCGGAGAGGACACCTCGAACCGGCCCCGTTCAGCGCGCCGGAGCCGCAAGCGCTGCTGCAAAACTGGCTTAGTG
AAACCGCGATGCGGCAATGGGAAAAGGATAATCAGTGTGATTTTGCGGTGCAGACGGACAGCGGCACGCGGCTGCGCGCC
AGTGCGTTCCGGCATGCGGACGGCGTTTCAATCGTTTTACGGCTGTTGCCGGAAATCTGTCCGCAGCTGGAGGAGCTTGG
CGTTCCGCAGGCCATCAACGAACTGCTGGCCGGAGAAAGCGGGCTGATTCTGGTGACGGGCGCCACCGGCAGTGGTAAAT
CCACCACCCTGGCGGCGATGGTCGACTACCTCAATCACCAGCGCGACGGGCATATTTTGACTCTTGAAGATCCGGTGGAG
TTCGTGCATCACAGCGAGCGATGTCTGATTCATCAGCGCGAAGTGGGGGAGCATTGCGGATCGTTTTCTGCGGCACTGCG
GGTGGCGTTGCGTCAGGATCCTGATGTGATTTTGCTCGGCGAGTTGCGCGACACCGAAACCATCCGTCTGGCGCTTACGG
CGGCGGAGACCGGGCATCTGGTGTTGTCGACGCTGCACACACGTGGCGCGGCACAGGCCATTGAAAGGCTGGTGGATGTG
TTTCCGGCCGGGGAAAAAGAGCAGGTGCGTAGCCAACTGTCCGGCAGCCTGTGCGCGGTGCTGGCGCAAAAACTGCTGCC
GGATAAACAGGGCGGACGCGTGGCGTTATATGAACTGTTGGTCAACACACCTGCTGTGGGCAGCCTGATTCGCGATGGTA
AAACCCATCAGTTGCCGGGCGTGATGCAGGCCGGGCAGCAAATGGGGATGATGACGTTTTCGCAGTGCTTCCAGCAGCGA
CAGGCGGCGGGTCGTCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.795

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.795

100

0.497
  pilT Pseudomonas stutzeri DSM 10701

47.59

100

0.485
  pilT Acinetobacter baylyi ADP1

47.59

100

0.485
  pilT Pseudomonas aeruginosa PAK

46.386

100

0.472
  pilT Neisseria meningitidis 8013

47.205

98.773

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Neisseria gonorrhoeae MS11

46.894

98.773

0.463
  pilT Acinetobacter nosocomialis M2

46.729

98.466

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.11

97.239

0.439
  pilU Vibrio cholerae strain A1552

41.925

98.773

0.414
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Acinetobacter baylyi ADP1

37.578

98.773

0.371