Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TC41_RS01975 Genome accession   NC_017167
Coordinates   420757..421797 (-) Length   346 a.a.
NCBI ID   WP_041694904.1    Uniprot ID   -
Organism   Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415757..426797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TC41_RS01945 (TC41_0416) - 416022..416564 (+) 543 WP_041694900.1 hypothetical protein -
  TC41_RS17195 - 416613..417266 (+) 654 WP_041694901.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TC41_RS01955 (TC41_0418) - 417462..418268 (+) 807 WP_014463294.1 hypothetical protein -
  TC41_RS01960 (TC41_0419) pilM 418320..419288 (+) 969 WP_041694903.1 type IV pilus biogenesis protein PilM -
  TC41_RS01965 (TC41_0420) - 419293..419901 (+) 609 WP_014463296.1 PilN domain-containing protein -
  TC41_RS01970 (TC41_0421) gspM 419898..420488 (+) 591 WP_014463297.1 type II secretion system protein GspM -
  TC41_RS01975 (TC41_0422) pilT 420757..421797 (-) 1041 WP_041694904.1 type IV pilus twitching motility protein PilT Machinery gene
  TC41_RS01980 (TC41_0423) - 421864..423015 (-) 1152 WP_014463299.1 RNA-guided endonuclease TnpB family protein -
  TC41_RS01985 (TC41_0424) - 422987..423556 (-) 570 Protein_382 IS607 family transposase -
  TC41_RS01990 (TC41_0425) - 423756..424541 (-) 786 WP_041694905.1 A24 family peptidase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38197.99 Da        Isoelectric Point: 6.9903

>NTDB_id=44982 TC41_RS01975 WP_041694904.1 420757..421797(-) (pilT) [Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1]
MQIDEILALAKEKEASDVHLSAGAPVTYRVHGDLEPMGAKLMPIDLETFARQMLSEDRWERFMEAGEIDFSYSIPGVARF
RVNAYRQRGSISIAARLIPQRIPTMEELGLPSVLKSLMNRPFGLVLVTGPTGSGKSTTLAAVLDAINQTQRKHIITLEDP
IEYLHTHKLSIIDQREIGQDTSGFAPALRAALRQDPDILLVGEMRDLETIATAITASETGHLVFATLHTSDAVQTVERII
DVFPPEQQGQIRIQLAGVLAGVVSQRLHRTRDGRGRVASFEVLVNTPAVANLIRSGKTHQIGAMMQTGRQFGMITMEMSI
RELVQQGKISPAILRQWQPAQLTQQS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=44982 TC41_RS01975 WP_041694904.1 420757..421797(-) (pilT) [Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1]
GTGCAAATCGACGAGATCTTAGCCTTGGCGAAAGAGAAAGAAGCTTCAGACGTTCATCTCTCAGCGGGTGCGCCGGTCAC
CTATCGCGTGCACGGTGATCTCGAGCCGATGGGTGCCAAGCTCATGCCTATCGATCTCGAGACCTTCGCTCGCCAAATGC
TCTCGGAGGATCGCTGGGAGCGCTTCATGGAGGCGGGCGAGATCGACTTTTCCTACAGCATTCCAGGTGTGGCGCGTTTC
CGCGTGAACGCGTATCGGCAGCGGGGCTCCATCAGCATCGCGGCACGGCTCATCCCACAGCGCATCCCGACGATGGAAGA
ACTCGGACTGCCAAGCGTGCTCAAATCGTTGATGAACCGGCCCTTCGGGCTGGTCCTCGTCACAGGCCCGACTGGGAGCG
GCAAGAGCACCACGCTCGCGGCGGTACTTGACGCCATCAACCAGACCCAGCGCAAGCACATCATCACCTTGGAGGACCCG
ATTGAGTACTTGCACACGCACAAGCTGTCGATCATCGACCAGCGCGAGATTGGCCAAGACACGTCGGGCTTTGCGCCCGC
GCTGCGCGCGGCACTCCGCCAGGACCCGGACATCCTGCTCGTCGGCGAGATGCGCGACCTCGAGACTATCGCGACGGCCA
TCACGGCGTCGGAAACCGGTCACTTGGTGTTTGCCACGCTGCATACGTCAGACGCGGTGCAGACGGTGGAGCGCATCATC
GACGTGTTTCCGCCCGAGCAGCAGGGGCAGATCCGCATCCAGCTCGCGGGCGTTTTGGCGGGCGTCGTCTCGCAGCGCCT
GCATCGCACGCGCGACGGGCGCGGGCGCGTGGCCTCGTTCGAGGTGCTCGTGAACACGCCCGCGGTGGCCAACCTCATCC
GCAGCGGCAAGACACACCAGATTGGCGCCATGATGCAGACGGGCCGCCAGTTCGGCATGATCACCATGGAGATGAGCATT
CGGGAGTTGGTGCAGCAAGGGAAGATCTCGCCAGCCATCCTGCGGCAGTGGCAGCCTGCGCAACTCACGCAGCAGTCGTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.682

94.509

0.488
  pilT Neisseria meningitidis 8013

49.408

97.688

0.483
  pilT Pseudomonas aeruginosa PAK

49.85

96.243

0.48
  pilT Acinetobacter baumannii strain A118

48.81

97.11

0.474
  pilT Acinetobacter nosocomialis M2

48.81

97.11

0.474
  pilT Acinetobacter baumannii D1279779

48.81

97.11

0.474
  pilT Neisseria gonorrhoeae MS11

48.665

97.399

0.474
  pilT Legionella pneumophila strain ERS1305867

48.048

96.243

0.462
  pilT Legionella pneumophila strain Lp02

48.048

96.243

0.462
  pilT Pseudomonas stutzeri DSM 10701

48.338

95.665

0.462
  pilT Acinetobacter baylyi ADP1

48.036

95.665

0.46
  pilT Vibrio cholerae strain A1552

46.364

95.376

0.442
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.364

95.376

0.442
  pilU Vibrio cholerae strain A1552

43.333

95.376

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.47

95.954

0.408
  pilU Acinetobacter baylyi ADP1

38.024

96.532

0.367

Multiple sequence alignment