Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HP437_RS22165 Genome accession   NZ_CP053927
Coordinates   4458005..4459027 (-) Length   340 a.a.
NCBI ID   WP_180547704.1    Uniprot ID   -
Organism   Serratia marcescens strain 11/2010     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4453005..4464027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HP437_RS22135 (HP437_22130) - 4453806..4454318 (+) 513 WP_180547702.1 SprT family zinc-dependent metalloprotease -
  HP437_RS22140 (HP437_22135) endA 4454420..4455115 (+) 696 WP_180547703.1 deoxyribonuclease I -
  HP437_RS22145 (HP437_22140) rsmE 4455185..4455916 (+) 732 WP_070913720.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HP437_RS22150 (HP437_22145) gshB 4455927..4456877 (+) 951 WP_047729854.1 glutathione synthase -
  HP437_RS22155 (HP437_22150) - 4457023..4457586 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  HP437_RS22160 (HP437_22155) ruvX 4457586..4458008 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  HP437_RS22165 (HP437_22160) pilT 4458005..4459027 (-) 1023 WP_180547704.1 type IV pilus twitching motility protein PilT Machinery gene
  HP437_RS22170 (HP437_22165) - 4459048..4459755 (+) 708 WP_060422654.1 YggS family pyridoxal phosphate-dependent enzyme -
  HP437_RS22175 (HP437_22170) proC 4459775..4460596 (+) 822 WP_060422650.1 pyrroline-5-carboxylate reductase -
  HP437_RS22180 (HP437_22175) - 4460628..4461182 (+) 555 WP_100397089.1 YggT family protein -
  HP437_RS22185 (HP437_22180) yggU 4461179..4461469 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  HP437_RS22190 (HP437_22185) - 4461487..4462080 (+) 594 WP_180547705.1 XTP/dITP diphosphatase -
  HP437_RS22195 (HP437_22190) hemW 4462073..4463215 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  HP437_RS22200 (HP437_22195) - 4463253..4463687 (-) 435 WP_180547706.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36375.72 Da        Isoelectric Point: 7.0670

>NTDB_id=449047 HP437_RS22165 WP_180547704.1 4458005..4459027(-) (pilT) [Serratia marcescens strain 11/2010]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLSAQQRESLRRLGQLDLALHRPGGE
RLRANVFQQSAGMSLALRRIVGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=449047 HP437_RS22165 WP_180547704.1 4458005..4459027(-) (pilT) [Serratia marcescens strain 11/2010]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCTCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAGTGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGCTCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGTCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCACCGGCCATCGTCCCGGCACTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAA
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATTCAACAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TGCGGCGTTGCGCGCTGCGTTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTCGCCACCATTCGGT
TGGCGCTCACTGCGGCGGAGACTGGTCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCTCAGGCGGTGGAAAGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGTGCCCAGTTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Neisseria meningitidis 8013

49.851

98.529

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.788

97.059

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368