Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HQK31_RS21155 Genome accession   NZ_CP053918
Coordinates   4392533..4393555 (-) Length   340 a.a.
NCBI ID   WP_369436559.1    Uniprot ID   -
Organism   Serratia marcescens strain LY1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4387533..4398555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQK31_RS21125 - 4388332..4388844 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  HQK31_RS21130 endA 4388946..4389641 (+) 696 WP_043141331.1 deoxyribonuclease I -
  HQK31_RS21135 rsmE 4389711..4390442 (+) 732 WP_323711781.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HQK31_RS21140 gshB 4390453..4391403 (+) 951 WP_043141334.1 glutathione synthase -
  HQK31_RS21145 - 4391551..4392114 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  HQK31_RS21150 ruvX 4392114..4392536 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  HQK31_RS21155 pilT 4392533..4393555 (-) 1023 WP_369436559.1 type IV pilus twitching motility protein PilT Machinery gene
  HQK31_RS21160 - 4393576..4394283 (+) 708 WP_060434540.1 YggS family pyridoxal phosphate-dependent enzyme -
  HQK31_RS21165 proC 4394303..4395124 (+) 822 WP_049296238.1 pyrroline-5-carboxylate reductase -
  HQK31_RS21170 - 4395156..4395710 (+) 555 WP_025304250.1 YggT family protein -
  HQK31_RS21175 yggU 4395707..4395997 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  HQK31_RS21180 - 4396015..4396608 (+) 594 WP_169541175.1 XTP/dITP diphosphatase -
  HQK31_RS21185 hemW 4396601..4397743 (+) 1143 WP_169541176.1 radical SAM family heme chaperone HemW -
  HQK31_RS21190 - 4397781..4398029 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  HQK31_RS21195 - 4398013..4398255 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36473.69 Da        Isoelectric Point: 7.0670

>NTDB_id=448937 HQK31_RS21155 WP_369436559.1 4392533..4393555(-) (pilT) [Serratia marcescens strain LY1]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGMSLALRRIAGQSPSLVELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=448937 HQK31_RS21155 WP_369436559.1 4392533..4393555(-) (pilT) [Serratia marcescens strain LY1]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCACAAGGCGCGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGTGGAGAG
CGGCTGCGTGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGTCGCCCTCGCT
TGTCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTCGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGAGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.552

98.529

0.488
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.444

100

0.447
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368