Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HQ398_RS20900 Genome accession   NZ_CP053882
Coordinates   4450333..4451367 (+) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas jandaei strain 3036     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4445333..4456367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ398_RS20870 (HQ398_20840) - 4446088..4447284 (+) 1197 WP_104016015.1 NupC/NupG family nucleoside CNT transporter -
  HQ398_RS20875 (HQ398_20845) - 4447387..4447806 (-) 420 WP_041209070.1 DUF4426 domain-containing protein -
  HQ398_RS20880 (HQ398_20850) yggU 4447836..4448135 (-) 300 WP_033114039.1 DUF167 family protein YggU -
  HQ398_RS20885 (HQ398_20855) - 4448135..4448686 (-) 552 WP_042031658.1 YggT family protein -
  HQ398_RS20890 (HQ398_20860) proC 4448698..4449525 (-) 828 WP_215806886.1 pyrroline-5-carboxylate reductase -
  HQ398_RS20895 (HQ398_20865) - 4449592..4450293 (-) 702 WP_215806888.1 YggS family pyridoxal phosphate-dependent enzyme -
  HQ398_RS20900 (HQ398_20870) pilT 4450333..4451367 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  HQ398_RS20905 (HQ398_20875) tapU 4451396..4452504 (+) 1109 Protein_4047 type IVa pilus ATPase TapU -
  HQ398_RS20910 (HQ398_20880) yaaA 4452552..4453325 (+) 774 WP_215806890.1 peroxide stress protein YaaA -
  HQ398_RS20915 (HQ398_20885) - 4453520..4453948 (+) 429 WP_033114045.1 hypothetical protein -
  HQ398_RS20920 (HQ398_20890) - 4454263..4454754 (-) 492 WP_104016021.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  HQ398_RS20925 (HQ398_20895) arfB 4454776..4455192 (-) 417 WP_033114047.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  HQ398_RS20930 (HQ398_20900) glnK 4455394..4455732 (+) 339 WP_033114048.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=448398 HQ398_RS20900 WP_005340354.1 4450333..4451367(+) (pilT) [Aeromonas jandaei strain 3036]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=448398 HQ398_RS20900 WP_005340354.1 4450333..4451367(+) (pilT) [Aeromonas jandaei strain 3036]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCGTCGGATCTACACCTGTCCGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACATCGGGAAGTGCACGCCCTCATTTACGACA
TCATGAACGATCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCTTTCCAGCAAGCTCGCGGTTCCGGCGCGGTATTTCGTACCATCCCCAGTACTGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGCGGTCTGGTACTGGTGACCGGGCCGACCGGTT
CCGGTAAGTCGACCACGCTTGCAGCCATGGTCAACTACATCAACGAGAACTTCCATCACCATATTCTCACCATCGAAGAC
CCCATCGAATTTGTGCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTTCACCGGGATACCAAGAGTTTCAGCAA
TGCCCTGCGCTCGGCACTGCGGGAAGACCCCGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACTGCCGCCGAAACCGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTTCCCGGCGCAGAGAAAGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGCGGTCGGGTGGCAGCCCACGAGATCATGATGGGGATCCCGGCGGTGCGTAACCTGATCC
GGGAGGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGTCTC
AAACAGCTGGTGAGTCGCGGCGTGGTGGCATCTCTCGACGCCAAGGCCAAGGCGGTTGACCCCAATAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407