Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CURT_RS07835 Genome accession   NZ_CP053832
Coordinates   1537148..1538230 (+) Length   360 a.a.
NCBI ID   WP_018712860.1    Uniprot ID   S5TT06
Organism   Campylobacter ureolyticus strain LMG 6451     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1532148..1543230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CURT_RS07800 (CURT_1513) - 1532718..1533623 (+) 906 WP_018712853.1 LysR substrate-binding domain-containing protein -
  CURT_RS07805 (CURT_1514) hisG 1533609..1534217 (-) 609 WP_018712854.1 ATP phosphoribosyltransferase -
  CURT_RS07810 (CURT_1515) - 1534214..1534846 (-) 633 WP_018712855.1 type III pantothenate kinase -
  CURT_RS07815 (CURT_1516) - 1534828..1535145 (-) 318 WP_018712856.1 hypothetical protein -
  CURT_RS07820 (CURT_1517) - 1535158..1536165 (-) 1008 WP_018712857.1 hypothetical protein -
  CURT_RS07825 (CURT_1518) - 1536152..1536730 (-) 579 WP_018712858.1 hypothetical protein -
  CURT_RS07830 (CURT_1519) gatC 1536854..1537135 (+) 282 WP_018712859.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CURT_RS07835 (CURT_1520) pilT 1537148..1538230 (+) 1083 WP_018712860.1 type IV pilus twitching motility protein PilT Machinery gene
  CURT_RS07840 (CURT_1521) - 1538231..1538875 (+) 645 WP_018712861.1 CvpA family protein -
  CURT_RS07845 (CURT_1522) - 1538844..1539326 (+) 483 WP_018712862.1 Fur family transcriptional regulator -
  CURT_RS07850 (CURT_1523) lysS 1539330..1540841 (+) 1512 WP_018712863.1 lysine--tRNA ligase -
  CURT_RS07855 (CURT_1524) - 1540844..1542088 (+) 1245 WP_018712864.1 serine hydroxymethyltransferase -
  CURT_RS07860 (CURT_1525) - 1542097..1542978 (+) 882 WP_018712865.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40163.06 Da        Isoelectric Point: 6.8355

>NTDB_id=448016 CURT_RS07835 WP_018712860.1 1537148..1538230(+) (pilT) [Campylobacter ureolyticus strain LMG 6451]
MEKSNIKTDIQTLLRVQINNDASDLHIVSRSAPQIRIDGKLVPLDMPNLTGTDIEHLCYAIITDAQKSELEEKKELDFAM
EIPNIGRFRGNYYYTMNGDLAAAFRKIPVDIPSLDDLRAPSIFKKLVKREKGMILVTGPTGSGKSTTLAAMLNEINLTER
KHIITVEDPVEFVHENKKCLFSHRNIGTDTHSYANALKYALREDPDIILIGEMRDRETISIAITAAETGHLVFATLHTNS
AMQTISRIVDSFDGSEQTQIRNMLSVSLSAIISQSLLPKIGKGRLAVHEILVNNHAIANLIREDKTQQIYSQMQLNQQET
GMVTQTQALLQALNKRLISKESALAYTTNRQELVKALGGE

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=448016 CURT_RS07835 WP_018712860.1 1537148..1538230(+) (pilT) [Campylobacter ureolyticus strain LMG 6451]
ATGGAAAAAAGCAATATAAAAACCGACATTCAAACCCTTTTAAGGGTGCAAATAAACAACGATGCAAGTGACCTTCACAT
AGTAAGTAGAAGTGCCCCACAAATTAGAATCGATGGAAAATTAGTTCCTCTTGATATGCCAAATTTAACAGGAACAGACA
TTGAACATCTTTGTTATGCAATTATTACAGATGCTCAAAAAAGCGAACTCGAAGAGAAAAAAGAGCTTGACTTTGCTATG
GAAATTCCAAATATAGGAAGGTTTAGAGGAAATTATTATTATACTATGAATGGTGATTTGGCAGCTGCATTTAGAAAAAT
TCCTGTTGATATTCCTTCTCTTGATGATTTAAGGGCTCCAAGTATTTTTAAAAAGCTAGTTAAAAGAGAAAAAGGGATGA
TTTTAGTAACTGGTCCTACAGGAAGTGGTAAATCAACTACTTTAGCAGCAATGTTAAATGAGATAAATTTAACTGAAAGA
AAACACATTATAACAGTCGAAGACCCAGTTGAGTTTGTCCATGAAAATAAAAAATGTCTTTTTTCTCATAGAAATATAGG
AACCGATACTCACTCTTATGCAAATGCTTTAAAGTATGCTCTTCGTGAGGACCCTGATATTATACTAATTGGTGAGATGA
GAGATAGAGAAACTATTTCAATCGCCATTACAGCAGCTGAAACAGGACACTTAGTTTTTGCTACACTTCACACAAATTCA
GCTATGCAAACAATCAGCCGTATAGTTGATAGTTTTGATGGAAGCGAACAAACCCAAATAAGAAATATGCTATCAGTTTC
ATTAAGTGCGATAATTTCACAATCTCTACTTCCAAAAATTGGAAAAGGACGACTTGCAGTTCATGAAATTTTGGTAAATA
ATCACGCTATTGCCAACCTAATAAGAGAAGACAAAACTCAACAAATTTATTCTCAAATGCAATTAAATCAACAAGAAACA
GGCATGGTAACTCAAACTCAAGCACTATTACAAGCGCTTAATAAAAGACTCATTTCAAAAGAAAGCGCTCTTGCTTATAC
TACAAATAGACAAGAGTTAGTTAAGGCTTTAGGGGGTGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S5TT06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.153

92.5

0.492
  pilT Vibrio cholerae strain A1552

53.153

92.5

0.492
  pilT Legionella pneumophila strain Lp02

51.462

95

0.489
  pilT Legionella pneumophila strain ERS1305867

51.462

95

0.489
  pilT Acinetobacter baylyi ADP1

50.742

93.611

0.475
  pilT Acinetobacter baumannii D1279779

50.746

93.056

0.472
  pilT Acinetobacter baumannii strain A118

50.746

93.056

0.472
  pilT Acinetobacter nosocomialis M2

50.746

93.056

0.472
  pilT Neisseria gonorrhoeae MS11

49.275

95.833

0.472
  pilT Neisseria meningitidis 8013

48.986

95.833

0.469
  pilT Pseudomonas stutzeri DSM 10701

50.448

93.056

0.469
  pilT Pseudomonas aeruginosa PAK

49.552

93.056

0.461
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.241

96.667

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.764

98.889

0.383
  pilU Acinetobacter baylyi ADP1

40

94.444

0.378
  pilU Vibrio cholerae strain A1552

41.463

91.111

0.378