Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSMK_RS12465 Genome accession   NC_017080
Coordinates   3101971..3103026 (-) Length   351 a.a.
NCBI ID   WP_014437970.1    Uniprot ID   -
Organism   Phycisphaera mikurensis NBRC 102666     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3096971..3108026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSMK_RS12445 (PSMK_25940) - 3098034..3098876 (-) 843 WP_014437966.1 C40 family peptidase -
  PSMK_RS12450 (PSMK_25950) - 3098882..3099922 (-) 1041 WP_053230171.1 fatty acid desaturase -
  PSMK_RS12455 (PSMK_25960) - 3100093..3100488 (-) 396 WP_014437968.1 YbaB/EbfC family nucleoid-associated protein -
  PSMK_RS12460 (PSMK_25970) - 3100606..3101829 (-) 1224 WP_014437969.1 type II secretion system F family protein -
  PSMK_RS12465 (PSMK_25980) pilT 3101971..3103026 (-) 1056 WP_014437970.1 type IV pilus twitching motility protein PilT Machinery gene
  PSMK_RS12470 (PSMK_25990) - 3103248..3104141 (-) 894 WP_014437971.1 hypothetical protein -
  PSMK_RS12475 (PSMK_26000) yidD 3104151..3104420 (-) 270 WP_014437972.1 membrane protein insertion efficiency factor YidD -
  PSMK_RS12480 (PSMK_26010) hemB 3104447..3105460 (-) 1014 WP_014437973.1 porphobilinogen synthase -
  PSMK_RS12485 (PSMK_26020) - 3105555..3106625 (+) 1071 WP_014437974.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39042.91 Da        Isoelectric Point: 6.0470

>NTDB_id=44764 PSMK_RS12465 WP_014437970.1 3101971..3103026(-) (pilT) [Phycisphaera mikurensis NBRC 102666]
MQLDTILKTALELDSSDVHLVAGHPPKCRIQTVMTTMDFPVITPESAAAMLKSMAGPEKLKQFEKIRDADFSYEVPNFAR
FRVNAHQQNGHIGITLRAIKTKIPALPNLNLPEVVSRLTYLPRGLVLVTGDTGSGKTTTLAAMLDAMNERYDKHIITLED
PIEYVLPSKRCLIEQRELGQDTPSFASGLRHVLRQDPDIILVGEMRDLETTAAAITAAETGHLVFSTLHTVNASHTVERI
VDMYPADQQNQIRSMLANTLQAVISQTLFKRLDQPGMVPASEIMLCTPAVRNLIRENRTYEIPNVIETNRPLGMQTLDNS
IAELYFNGMIDRDSALSQAAYPEKLERQIAA

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=44764 PSMK_RS12465 WP_014437970.1 3101971..3103026(-) (pilT) [Phycisphaera mikurensis NBRC 102666]
ATGCAGCTGGACACCATCCTCAAGACCGCCCTGGAGCTGGACAGCTCCGACGTCCACCTGGTCGCGGGCCACCCGCCCAA
GTGCCGGATCCAGACGGTGATGACCACCATGGACTTCCCGGTGATCACGCCGGAGTCCGCGGCGGCGATGCTCAAGTCGA
TGGCCGGCCCCGAGAAGCTCAAGCAGTTCGAGAAGATCCGCGACGCGGACTTCTCCTACGAGGTGCCCAACTTCGCTCGC
TTCCGCGTCAACGCCCACCAGCAAAACGGCCACATCGGCATCACGCTGCGGGCGATCAAGACCAAGATCCCGGCGCTGCC
GAACCTCAACCTGCCCGAGGTCGTCTCGCGGCTGACGTACCTGCCCCGCGGCCTCGTGCTCGTGACCGGCGACACCGGCT
CGGGCAAGACGACCACGCTGGCGGCGATGCTCGACGCGATGAACGAGCGCTACGACAAGCACATCATCACCCTCGAGGAT
CCGATCGAGTACGTCCTGCCCTCGAAGCGGTGCCTGATCGAGCAACGCGAGCTCGGGCAGGACACCCCCAGCTTCGCCAG
CGGGCTGCGGCACGTGCTGCGGCAGGACCCCGACATCATCCTCGTCGGGGAGATGCGCGACCTGGAGACGACCGCCGCGG
CGATCACCGCCGCCGAGACGGGCCACCTGGTCTTCTCGACGCTGCACACGGTCAACGCCTCCCACACCGTCGAGCGCATC
GTCGACATGTACCCCGCCGACCAGCAGAACCAGATCCGCTCGATGCTGGCGAACACGCTGCAGGCGGTGATCTCGCAGAC
GCTCTTCAAGCGGCTCGACCAGCCGGGCATGGTGCCCGCCAGCGAGATCATGCTCTGCACGCCCGCCGTCCGGAACCTGA
TCCGCGAGAACCGCACGTACGAGATCCCCAACGTGATCGAGACGAACCGGCCGCTGGGCATGCAGACGCTCGACAACTCC
ATCGCGGAGCTCTACTTCAACGGAATGATCGACCGCGACTCGGCCCTCTCCCAGGCCGCGTACCCCGAGAAGCTCGAGCG
TCAGATCGCCGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

48.986

98.291

0.481
  pilT Pseudomonas aeruginosa PAK

48.406

98.291

0.476
  pilT Acinetobacter baumannii D1279779

47.399

98.575

0.467
  pilT Acinetobacter baumannii strain A118

47.399

98.575

0.467
  pilT Acinetobacter nosocomialis M2

47.399

98.575

0.467
  pilT Acinetobacter baylyi ADP1

46.821

98.575

0.462
  pilT Neisseria meningitidis 8013

46.377

98.291

0.456
  pilT Neisseria gonorrhoeae MS11

46.087

98.291

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.199

97.436

0.45
  pilT Legionella pneumophila strain ERS1305867

45.075

95.442

0.43
  pilT Legionella pneumophila strain Lp02

45.075

95.442

0.43
  pilT Vibrio cholerae strain A1552

45.317

94.302

0.427
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.317

94.302

0.427
  pilU Acinetobacter baylyi ADP1

36.857

99.715

0.368
  pilU Vibrio cholerae strain A1552

37.758

96.581

0.365

Multiple sequence alignment