Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FX982_RS06305 Genome accession   NZ_CP053746
Coordinates   1374194..1375228 (-) Length   344 a.a.
NCBI ID   WP_065991152.1    Uniprot ID   -
Organism   Pseudomonas graminis strain PgKB30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1369194..1380228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX982_RS06280 (FX982_01264) pyrR 1369705..1370226 (+) 522 WP_172610020.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  FX982_RS06285 (FX982_01265) - 1370239..1371243 (+) 1005 WP_172610021.1 aspartate carbamoyltransferase catalytic subunit -
  FX982_RS06290 (FX982_01266) - 1371240..1372511 (+) 1272 WP_172610022.1 dihydroorotase -
  FX982_RS06295 (FX982_01267) - 1372725..1373141 (-) 417 WP_172610023.1 TM2 domain-containing protein -
  FX982_RS06300 (FX982_01268) - 1373392..1374123 (+) 732 WP_172610024.1 C40 family peptidase -
  FX982_RS06305 (FX982_01269) pilT 1374194..1375228 (-) 1035 WP_065991152.1 type IV pilus twitching motility protein PilT Machinery gene
  FX982_RS06310 (FX982_01270) - 1375283..1375969 (+) 687 WP_172610025.1 YggS family pyridoxal phosphate-dependent enzyme -
  FX982_RS06315 (FX982_01271) proC 1375994..1376812 (+) 819 WP_172610026.1 pyrroline-5-carboxylate reductase -
  FX982_RS06320 (FX982_01272) - 1376823..1377413 (+) 591 WP_065991147.1 YggT family protein -
  FX982_RS06325 (FX982_01273) - 1377475..1378470 (-) 996 WP_172610027.1 IS110 family transposase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37926.48 Da        Isoelectric Point: 6.5802

>NTDB_id=446816 FX982_RS06305 WP_065991152.1 1374194..1375228(-) (pilT) [Pseudomonas graminis strain PgKB30]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDQKEVQALIYDIMNDRQRKDFEEDLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMVDYLNSNKHHHILTIED
PIEFVHESKKSLINQREVHRDTHGFAEALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKSIDRI
VDVFPAQEKSMIRSMLSESLHAIVSQALLKKVGGGRVAAHEIMIGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLISKGLITRESAREKAKTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=446816 FX982_RS06305 WP_065991152.1 1374194..1375228(-) (pilT) [Pseudomonas graminis strain PgKB30]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAGCAAGGCGCGTCGGACTTGCATCTCTCTGCCGGCCTGCCACCGAT
GATTCGCGTCGATGGTGATGTGCGGCGGATAAACCTGCCCGCACTGGACCAGAAAGAGGTTCAGGCGCTGATCTACGACA
TCATGAACGACAGGCAGCGTAAGGACTTCGAGGAGGACCTGGAGACAGACTTTTCCTTCGAAGTCCCAGGCGTGGCGCGC
TTTCGGGTCAACGCGTTCAACCAGAATCGCGGCGCCGGCGCGGTGTTTCGGACCATTCCGTCGAAGGTCCTGAGCATGGA
AGAGCTGGGCATGGGCGAAGTGTTTCGCAAGATTACCGACGTGCCCCGCGGCCTGGTGTTGGTCACCGGTCCGACCGGTT
CAGGCAAGTCGACCACCCTCGCGGCCATGGTCGATTACCTGAACAGCAACAAGCATCACCACATCCTCACCATTGAAGAC
CCGATCGAATTCGTACACGAGTCCAAAAAAAGCCTGATCAACCAGCGCGAAGTCCATCGCGACACCCACGGCTTTGCCGA
AGCGCTGCGGTCGGCGCTGCGAGAAGACCCTGACGTGATTCTGGTGGGTGAGCTGCGCGACCTTGAGACCATCCGCCTGG
CCCTGACGGCGGCAGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCACGTCCGCTGCCAAGAGCATCGACCGTATC
GTCGATGTGTTCCCGGCGCAGGAAAAGTCGATGATCCGCTCAATGCTTTCCGAGTCGCTTCACGCCATCGTGTCTCAGGC
GCTGCTGAAGAAGGTCGGCGGAGGCAGGGTCGCTGCTCACGAGATCATGATCGGCACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCGATTCAGACCGGGGGGTCGCTGGGGATGCAGACGCTGGACATGTGCCTG
AAGGATCTGATCAGCAAGGGGCTGATCACGCGTGAGAGTGCGCGGGAGAAGGCGAAAACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Vibrio cholerae strain A1552

38.841

100

0.39