Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HPY04_RS11605 Genome accession   NZ_CP053744
Coordinates   2586885..2587922 (-) Length   345 a.a.
NCBI ID   WP_175249131.1    Uniprot ID   -
Organism   Vibrio cholerae strain SA3G     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2581885..2592922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY04_RS11580 (HPY04_11590) - 2582356..2582919 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  HPY04_RS11585 (HPY04_11595) ruvX 2583000..2583422 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  HPY04_RS11590 (HPY04_11600) tyrS 2583460..2584740 (-) 1281 WP_175249129.1 tyrosine--tRNA ligase -
  HPY04_RS11595 (HPY04_11605) - 2584849..2585646 (-) 798 WP_175249130.1 LuxR C-terminal-related transcriptional regulator -
  HPY04_RS11600 (HPY04_11610) pilU 2585764..2586870 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HPY04_RS11605 (HPY04_11615) pilT 2586885..2587922 (-) 1038 WP_175249131.1 type IV pilus twitching motility protein PilT Machinery gene
  HPY04_RS11610 (HPY04_11620) - 2587948..2588658 (+) 711 WP_175249132.1 YggS family pyridoxal phosphate-dependent enzyme -
  HPY04_RS11615 (HPY04_11625) proC 2588711..2589529 (+) 819 WP_053034663.1 pyrroline-5-carboxylate reductase -
  HPY04_RS11620 (HPY04_11630) - 2589583..2590140 (+) 558 WP_175249133.1 YggT family protein -
  HPY04_RS11625 (HPY04_11635) yggU 2590140..2590430 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  HPY04_RS11630 (HPY04_11640) - 2590476..2590907 (+) 432 WP_001233675.1 DUF4426 domain-containing protein -
  HPY04_RS11635 (HPY04_11645) - 2590977..2591579 (+) 603 WP_000725008.1 XTP/dITP diphosphatase -
  HPY04_RS11640 (HPY04_11650) hemW 2591573..2592748 (+) 1176 WP_175249134.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38263.89 Da        Isoelectric Point: 6.6672

>NTDB_id=446798 HPY04_RS11605 WP_175249131.1 2586885..2587922(-) (pilT) [Vibrio cholerae strain SA3G]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFMHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=446798 HPY04_RS11605 WP_175249131.1 2586885..2587922(-) (pilT) [Vibrio cholerae strain SA3G]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGTTAGA
GCAACTGGATGCTCCAGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACGACGTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTATGCACAGCAACAATAAATGCTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTTTGGTTGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCGACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCTCATGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCACGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.71

100

0.997
  pilT Vibrio cholerae strain A1552

99.71

100

0.997
  pilT Acinetobacter baumannii D1279779

71.429

97.391

0.696
  pilT Acinetobacter baumannii strain A118

71.429

97.391

0.696
  pilT Acinetobacter nosocomialis M2

71.131

97.391

0.693
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.294

94.783

0.496
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377