Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7994_RS01995 Genome accession   NZ_CP065152
Coordinates   394272..395252 (-) Length   326 a.a.
NCBI ID   WP_196316090.1    Uniprot ID   -
Organism   Escherichia coli O150:H6 strain FEX669     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 389272..400252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7994_RS01965 (H7994_01965) yggI 389937..390434 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  H7994_RS01970 (H7994_01970) endA 390529..391236 (+) 708 WP_001327408.1 deoxyribonuclease I -
  H7994_RS01975 (H7994_01975) rsmE 391316..392047 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H7994_RS01980 (H7994_01980) gshB 392060..393010 (+) 951 WP_000593259.1 glutathione synthase -
  H7994_RS01985 (H7994_01985) yqgE 393119..393682 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  H7994_RS01990 (H7994_01990) ruvX 393682..394098 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  H7994_RS01995 (H7994_01995) pilT 394272..395252 (-) 981 WP_196316090.1 type IV pilus twitching motility protein PilT Machinery gene
  H7994_RS02000 (H7994_02000) yggS 395270..395974 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  H7994_RS02005 (H7994_02005) yggT 395992..396558 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  H7994_RS02010 (H7994_02010) yggU 396555..396845 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  H7994_RS02015 (H7994_02015) rdgB 396853..397446 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  H7994_RS02020 (H7994_02020) hemW 397439..398575 (+) 1137 WP_000239953.1 radical SAM family heme chaperone HemW -
  H7994_RS02025 (H7994_02025) yggM 398640..399647 (-) 1008 WP_000745232.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36004.25 Da        Isoelectric Point: 5.7990

>NTDB_id=445648 H7994_RS01995 WP_196316090.1 394272..395252(-) (pilT) [Escherichia coli O150:H6 strain FEX669]
MNMEEIVALSVKHNVSDLHLCSAWPARWRICGRMEAAPFDAPDVEELLREWLDDEQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=445648 H7994_RS01995 WP_196316090.1 394272..395252(-) (pilT) [Escherichia coli O150:H6 strain FEX669]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTTGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGAGCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365