Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IPT86_RS19195 Genome accession   NZ_CP064847
Coordinates   3979972..3980952 (-) Length   326 a.a.
NCBI ID   WP_075862143.1    Uniprot ID   -
Organism   Escherichia coli strain 20     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3974972..3985952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IPT86_RS19165 (IPT86_19165) yggI 3975598..3976095 (+) 498 WP_023908971.1 SprT family zinc-dependent metalloprotease -
  IPT86_RS19170 (IPT86_19170) endA 3976190..3976897 (+) 708 WP_000286500.1 deoxyribonuclease I -
  IPT86_RS19175 (IPT86_19175) rsmE 3976977..3977708 (+) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IPT86_RS19180 (IPT86_19180) gshB 3977721..3978671 (+) 951 WP_075862144.1 glutathione synthase -
  IPT86_RS19185 (IPT86_19185) yqgE 3978780..3979343 (+) 564 WP_001339287.1 YqgE/AlgH family protein -
  IPT86_RS19190 (IPT86_19190) ruvX 3979343..3979759 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  IPT86_RS19195 (IPT86_19195) pilT 3979972..3980952 (-) 981 WP_075862143.1 type IV pilus twitching motility protein PilT Machinery gene
  IPT86_RS19200 (IPT86_19200) yggS 3980970..3981674 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  IPT86_RS19205 (IPT86_19205) yggT 3981692..3982258 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  IPT86_RS19210 (IPT86_19210) yggU 3982255..3982545 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  IPT86_RS19215 (IPT86_19215) rdgB 3982553..3983146 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  IPT86_RS19220 (IPT86_19220) hemW 3983139..3984275 (+) 1137 WP_075862142.1 radical SAM family heme chaperone HemW -
  IPT86_RS19225 (IPT86_19225) - 3984588..3985574 (+) 987 WP_001564022.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35929.12 Da        Isoelectric Point: 5.5860

>NTDB_id=445308 IPT86_RS19195 WP_075862143.1 3979972..3980952(-) (pilT) [Escherichia coli strain 20]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPIE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=445308 IPT86_RS19195 WP_075862143.1 3979972..3980952(-) (pilT) [Escherichia coli strain 20]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAAAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTATTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTAGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCAGTTTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCAGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371