Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HJG54_RS02770 Genome accession   NZ_CP053586
Coordinates   772005..773120 (+) Length   371 a.a.
NCBI ID   WP_316433223.1    Uniprot ID   -
Organism   Leptolyngbya sp. NK1-12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 767005..778120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HJG54_RS02755 (HJG54_02765) - 768181..768453 (+) 273 WP_316433220.1 hypothetical protein -
  HJG54_RS02760 (HJG54_02770) - 768440..769012 (-) 573 WP_316433221.1 hypothetical protein -
  HJG54_RS02765 (HJG54_02775) pilB/pilB1 769883..771886 (+) 2004 WP_316433222.1 GspE/PulE family protein Machinery gene
  HJG54_RS02770 (HJG54_02780) pilT 772005..773120 (+) 1116 WP_316433223.1 type IV pilus twitching motility protein PilT Machinery gene
  HJG54_RS02775 (HJG54_02785) - 773255..774487 (+) 1233 WP_316433224.1 type II secretion system F family protein -
  HJG54_RS02780 (HJG54_02790) - 774821..775453 (+) 633 WP_051925871.1 hypothetical protein -
  HJG54_RS02785 (HJG54_02795) - 775797..776006 (+) 210 WP_316433225.1 DUF2997 domain-containing protein -
  HJG54_RS02790 (HJG54_02800) - 776069..776455 (+) 387 WP_036001565.1 DUF1257 domain-containing protein -
  HJG54_RS02795 (HJG54_02805) - 776527..776979 (+) 453 WP_316433227.1 ferredoxin -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40728.13 Da        Isoelectric Point: 6.7589

>NTDB_id=445267 HJG54_RS02770 WP_316433223.1 772005..773120(+) (pilT) [Leptolyngbya sp. NK1-12]
MELMIEDLMEQLIEMGGSDMHLSAGLPPYFRISGHLTPIGDEPMTAEQCQRLIFSMLNNTQRKNLEQNWELDCSYGVKGL
ARFRVNVYKDRGTYAACLRALSSKIPSFDKLGLPDVVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTI
EDPIEFVYEPIKSLIHQRQLGEDTKSFANALRAALREDPDIILVGEMRDLETISLAISAAETGHLVFATLHTSSAAQTVD
RMIDVFPAERQQQVRVQLSNSLVAVFSQTLVAKKNPKPGEFGRAMAQEIMVVTPAIANLIREGKTSQIYSAIQTGGKLGM
VTLEKVLADLYKAGTISFEAAMSKTSRPDELQRLIGNAPMPAKPGMPAAAK

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=445267 HJG54_RS02770 WP_316433223.1 772005..773120(+) (pilT) [Leptolyngbya sp. NK1-12]
ATGGAGCTAATGATCGAAGACTTGATGGAACAACTAATCGAAATGGGCGGGTCTGATATGCACCTGTCGGCGGGTCTGCC
TCCCTATTTCCGGATTAGCGGTCATCTCACCCCAATTGGCGATGAACCAATGACGGCAGAACAGTGCCAGCGGCTGATCT
TCAGCATGCTCAACAACACTCAGCGAAAAAACCTAGAGCAAAACTGGGAGCTAGACTGTTCCTACGGCGTCAAGGGCTTA
GCTCGTTTTCGGGTAAATGTCTATAAAGATCGAGGCACCTATGCCGCCTGCTTACGGGCGCTTAGCTCCAAAATTCCCAG
CTTCGACAAACTCGGTCTGCCGGATGTGGTGCGGGAAATGTCAGAAAAGCCACGCGGGCTGATTCTGGTAACAGGGCCAA
CCGGTTCGGGTAAAACCACAACGCTGGCCGCCATGATCGACCTGATCAACCGCACTCGGGCCGAACACATTCTTACCATC
GAAGACCCCATTGAATTTGTCTACGAGCCGATCAAAAGCCTGATTCACCAGCGCCAGTTGGGCGAAGATACCAAGAGCTT
TGCCAATGCGCTGCGAGCTGCCCTACGGGAAGACCCGGATATTATTCTGGTGGGGGAAATGCGGGACCTGGAAACGATTT
CGCTCGCCATCTCCGCCGCAGAAACGGGCCACTTGGTCTTTGCTACGCTGCACACTAGCTCTGCTGCCCAAACCGTAGAC
CGGATGATCGACGTATTTCCAGCAGAGCGGCAGCAGCAAGTGCGGGTCCAGCTCTCGAACTCGCTAGTTGCCGTCTTTAG
CCAAACCTTAGTCGCCAAGAAAAACCCCAAGCCTGGTGAATTTGGGCGGGCAATGGCACAAGAGATCATGGTTGTCACGC
CCGCAATTGCTAACCTGATTCGGGAAGGCAAAACCTCACAAATCTACTCGGCGATCCAAACTGGCGGCAAATTGGGCATG
GTGACGCTAGAAAAGGTGCTGGCCGATCTGTACAAAGCAGGCACGATTTCCTTTGAAGCGGCCATGTCCAAAACCTCTCG
CCCGGATGAATTGCAGCGACTGATTGGCAACGCCCCCATGCCCGCCAAGCCCGGTATGCCTGCCGCTGCTAAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.179

93.531

0.507
  pilT Pseudomonas stutzeri DSM 10701

53.602

93.531

0.501
  pilT Acinetobacter baylyi ADP1

52.45

93.531

0.491
  pilT Legionella pneumophila strain Lp02

52.464

92.992

0.488
  pilT Legionella pneumophila strain ERS1305867

52.464

92.992

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.656

100

0.488
  pilT Acinetobacter nosocomialis M2

51.297

93.531

0.48
  pilT Acinetobacter baumannii strain A118

51.297

93.531

0.48
  pilT Acinetobacter baumannii D1279779

51.297

93.531

0.48
  pilT Neisseria meningitidis 8013

49.284

94.07

0.464
  pilT Neisseria gonorrhoeae MS11

50.737

91.375

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.412

91.644

0.453
  pilT Vibrio cholerae strain A1552

49.412

91.644

0.453
  pilU Acinetobacter baylyi ADP1

37.673

97.305

0.367
  pilU Pseudomonas stutzeri DSM 10701

38.746

94.609

0.367