Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HJG54_RS02765 Genome accession   NZ_CP053586
Coordinates   769883..771886 (+) Length   667 a.a.
NCBI ID   WP_316433222.1    Uniprot ID   -
Organism   Leptolyngbya sp. NK1-12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 764883..776886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HJG54_RS02740 (HJG54_02750) - 765105..765563 (-) 459 WP_316433217.1 DUF29 domain-containing protein -
  HJG54_RS02745 (HJG54_02755) - 765557..765769 (+) 213 WP_316433218.1 hypothetical protein -
  HJG54_RS02750 (HJG54_02760) - 765807..768017 (+) 2211 WP_316433219.1 pentapeptide repeat-containing protein -
  HJG54_RS02755 (HJG54_02765) - 768181..768453 (+) 273 WP_316433220.1 hypothetical protein -
  HJG54_RS02760 (HJG54_02770) - 768440..769012 (-) 573 WP_316433221.1 hypothetical protein -
  HJG54_RS02765 (HJG54_02775) pilB/pilB1 769883..771886 (+) 2004 WP_316433222.1 GspE/PulE family protein Machinery gene
  HJG54_RS02770 (HJG54_02780) pilT 772005..773120 (+) 1116 WP_316433223.1 type IV pilus twitching motility protein PilT Machinery gene
  HJG54_RS02775 (HJG54_02785) - 773255..774487 (+) 1233 WP_316433224.1 type II secretion system F family protein -
  HJG54_RS02780 (HJG54_02790) - 774821..775453 (+) 633 WP_051925871.1 hypothetical protein -
  HJG54_RS02785 (HJG54_02795) - 775797..776006 (+) 210 WP_316433225.1 DUF2997 domain-containing protein -
  HJG54_RS02790 (HJG54_02800) - 776069..776455 (+) 387 WP_036001565.1 DUF1257 domain-containing protein -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74789.57 Da        Isoelectric Point: 4.9515

>NTDB_id=445266 HJG54_RS02765 WP_316433222.1 769883..771886(+) (pilB/pilB1) [Leptolyngbya sp. NK1-12]
MANSSSQRRALIVQHNFSPFSNKLVQSGFVNNEQMQQALIESRKSGRPLTDVLAMITGQQLPPDLLRQYKKQQLFELKIL
YGVESLDPEINQINASQLSQLIETLIPVDICRRYRLIPLSRTDGEPPSVLVAMVNPDDLAAQDDLNRILRPQGLTLQRMV
ITAEDYQRLISQYLDEQVERQKQLEFESKVDVKSDIENLDYLDLEEPADDLEVNLDAALEDADAAPIITLANKILAKALQ
DGISDIHIEPQEDYLRIRFRKDGVLRQGLDPLPKKIIPAVTARFKIMANLDIAERRVPQDGRIRRLFDGRKVDFRVNTLP
SRYGEKVVLRILDNSATQLGLDKLITDPETLHIVKEMVSRPFGLILVTGPTGSGKTTTLYSALAERNDPGINISTVEDPI
EYSLPGLTQVQVLREKGMDFASVLRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIARLEEMGI
ESFMVSSSLIGVLAQRLMRRVCPDCRIPYNPSSEELARFGLSSSRDAEITFYRANTLTNEEIHQASVENSLCPTCKGSGY
KGRCGVYEVMRVTERMQTLITEGAPTERIKEAAVEEGMKTLLAYSLNLVRQGATTLEEVERVTFTDTGLEAELKAKRKLS
LTCRCCGAELRQEWLDCPYCTTPRFQD

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=445266 HJG54_RS02765 WP_316433222.1 769883..771886(+) (pilB/pilB1) [Leptolyngbya sp. NK1-12]
ATGGCTAATTCCTCGTCGCAGCGGCGCGCCCTGATCGTTCAACACAATTTTTCTCCCTTCAGCAACAAACTGGTTCAGTC
GGGCTTTGTCAACAATGAACAGATGCAGCAAGCGCTGATTGAAAGTCGCAAGTCAGGTCGGCCACTCACCGATGTGCTGG
CGATGATCACCGGGCAACAGTTGCCGCCTGATTTATTACGTCAATATAAGAAGCAACAGCTATTTGAACTAAAAATTCTC
TATGGCGTCGAGTCGCTGGACCCAGAGATTAACCAGATCAATGCCAGCCAGCTCAGTCAGCTGATCGAGACCTTGATCCC
GGTTGATATTTGCCGCCGCTATCGTCTGATTCCACTTAGCCGCACCGACGGAGAACCGCCCTCGGTGTTGGTGGCAATGG
TGAATCCCGATGATCTAGCAGCCCAGGACGACCTGAACCGGATTCTGCGCCCGCAGGGATTGACACTGCAGCGAATGGTA
ATTACCGCAGAGGATTACCAGCGCCTGATTTCCCAGTATCTAGACGAGCAGGTTGAACGACAAAAGCAGCTGGAGTTTGA
ATCCAAAGTTGATGTCAAATCCGATATTGAAAACCTGGATTATCTCGACTTAGAAGAGCCGGCAGATGATCTGGAAGTCA
ACCTGGATGCGGCACTCGAGGATGCAGATGCAGCTCCCATCATTACATTGGCAAATAAGATCCTGGCGAAAGCGCTCCAG
GACGGTATTTCAGATATCCACATCGAGCCGCAAGAAGACTATCTGCGGATTCGGTTCCGCAAAGACGGGGTATTGCGCCA
AGGGCTAGATCCACTGCCGAAAAAGATTATTCCAGCGGTAACGGCCCGTTTTAAGATCATGGCCAACCTCGATATTGCCG
AACGGCGCGTGCCCCAAGATGGCCGGATTCGACGTCTGTTTGACGGTCGCAAAGTAGACTTCCGGGTCAATACCCTACCC
AGCCGCTACGGTGAAAAGGTCGTACTGCGGATTCTGGACAACTCGGCTACCCAGCTCGGTTTGGATAAGCTGATCACTGA
CCCCGAAACGCTGCATATCGTCAAAGAAATGGTGTCGCGTCCGTTTGGGCTGATTCTGGTGACGGGTCCAACCGGTTCCG
GAAAAACCACGACGCTCTATTCGGCCTTGGCCGAGCGCAACGATCCGGGTATCAACATCAGTACGGTAGAGGATCCGATT
GAATATTCGCTACCCGGTCTGACTCAGGTGCAGGTGCTGCGCGAAAAAGGCATGGACTTCGCCTCGGTACTGCGAGCTTT
CCTGCGGCAAGACCCGGATGTGATTCTGGTGGGTGAGACCCGCGACAAGGAAACTGCCAAAACCGCCATTGAAGCCGCCT
TAACCGGCCACTTGGTTTTGACCACCCTGCATACCAATGATGCTGCCAGCGCTATTGCTCGGCTCGAAGAAATGGGCATT
GAGTCCTTTATGGTCTCCAGCTCGTTGATTGGAGTACTAGCTCAGCGACTGATGCGCCGTGTTTGTCCTGATTGCCGCAT
TCCCTACAATCCCAGCTCCGAAGAGCTGGCCCGCTTTGGTTTGTCGAGTTCCCGCGATGCAGAAATCACCTTCTATCGAG
CCAATACCCTCACTAACGAGGAAATTCATCAAGCCAGCGTCGAGAATAGTCTTTGCCCCACCTGCAAAGGATCGGGATAT
AAAGGCCGCTGTGGGGTCTACGAGGTGATGCGCGTGACTGAACGCATGCAGACTCTGATTACCGAAGGCGCTCCCACCGA
GCGGATCAAGGAAGCTGCCGTCGAAGAAGGCATGAAAACCCTGTTGGCCTATAGCTTGAACCTAGTGCGTCAGGGGGCAA
CCACTCTGGAAGAAGTGGAGCGGGTCACCTTTACCGATACTGGTCTGGAGGCGGAGCTGAAAGCCAAACGTAAATTGTCG
CTTACCTGTCGCTGTTGTGGTGCTGAGCTGAGGCAGGAATGGCTGGACTGCCCCTACTGCACAACGCCCAGATTTCAGGA
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-178)

Type II/IV secretion system protein (T2SSE)

(233-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.214

99.4

0.658
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.516

92.954

0.367