Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HF677_RS03890 Genome accession   NZ_CP053573
Coordinates   810753..811733 (+) Length   326 a.a.
NCBI ID   WP_095716017.1    Uniprot ID   -
Organism   Citrobacter sp. TSA-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 805753..816733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF677_RS03860 (HF677_003860) - 806129..807115 (-) 987 WP_053389413.1 TRAP transporter substrate-binding protein -
  HF677_RS03865 (HF677_003865) hemW 807429..808565 (-) 1137 WP_019077663.1 radical SAM family heme chaperone HemW -
  HF677_RS03870 (HF677_003870) - 808558..809151 (-) 594 WP_016154402.1 XTP/dITP diphosphatase -
  HF677_RS03875 (HF677_003875) yggU 809159..809449 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  HF677_RS03880 (HF677_003880) - 809446..810012 (-) 567 WP_016154401.1 YggT family protein -
  HF677_RS03885 (HF677_003885) - 810031..810735 (-) 705 WP_016154400.1 YggS family pyridoxal phosphate-dependent enzyme -
  HF677_RS03890 (HF677_003890) pilT 810753..811733 (+) 981 WP_095716017.1 type IV pilus twitching motility protein PilT Machinery gene
  HF677_RS03895 (HF677_003895) ruvX 811730..812146 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  HF677_RS03900 (HF677_003900) - 812146..812709 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  HF677_RS03905 (HF677_003905) gshB 812886..813833 (-) 948 WP_047414456.1 glutathione synthase -
  HF677_RS03910 (HF677_003910) rsmE 813846..814577 (-) 732 WP_080859198.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HF677_RS03915 (HF677_003915) endA 814653..815360 (-) 708 WP_016154392.1 deoxyribonuclease I -
  HF677_RS03920 (HF677_003920) - 815455..815952 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35827.95 Da        Isoelectric Point: 6.1705

>NTDB_id=445224 HF677_RS03890 WP_095716017.1 810753..811733(+) (pilT) [Citrobacter sp. TSA-1]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLCGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQVKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=445224 HF677_RS03890 WP_095716017.1 810753..811733(+) (pilT) [Citrobacter sp. TSA-1]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACCCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTAACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGTG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGTGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGTTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Neisseria gonorrhoeae MS11

45.732

100

0.46
  pilT Neisseria meningitidis 8013

45.732

100

0.46
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.855

97.546

0.457
  pilT Legionella pneumophila strain Lp02

46.855

97.546

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365