Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F0335_RS20525 Genome accession   NZ_CP053572
Coordinates   4284355..4285377 (-) Length   340 a.a.
NCBI ID   WP_063988709.1    Uniprot ID   -
Organism   Serratia marcescens strain S7.1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4279355..4290377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0335_RS20495 (F0335_20515) - 4280157..4280669 (+) 513 WP_107227031.1 SprT family zinc-dependent metalloprotease -
  F0335_RS20500 (F0335_20520) endA 4280771..4281466 (+) 696 WP_063988712.1 deoxyribonuclease I -
  F0335_RS20505 (F0335_20525) rsmE 4281536..4282267 (+) 732 WP_049194742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F0335_RS20510 (F0335_20530) gshB 4282278..4283228 (+) 951 WP_063988711.1 glutathione synthase -
  F0335_RS20515 (F0335_20535) - 4283373..4283936 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  F0335_RS20520 (F0335_20540) ruvX 4283936..4284358 (+) 423 WP_063988710.1 Holliday junction resolvase RuvX -
  F0335_RS20525 (F0335_20545) pilT 4284355..4285377 (-) 1023 WP_063988709.1 type IV pilus twitching motility protein PilT Machinery gene
  F0335_RS20530 (F0335_20550) - 4285398..4286105 (+) 708 WP_149559971.1 YggS family pyridoxal phosphate-dependent enzyme -
  F0335_RS20535 (F0335_20555) proC 4286125..4286946 (+) 822 WP_063988707.1 pyrroline-5-carboxylate reductase -
  F0335_RS20540 (F0335_20560) - 4286978..4287532 (+) 555 WP_063988706.1 YggT family protein -
  F0335_RS20545 (F0335_20565) yggU 4287529..4287819 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  F0335_RS20550 (F0335_20570) - 4287837..4288430 (+) 594 WP_149559972.1 XTP/dITP diphosphatase -
  F0335_RS20555 (F0335_20575) hemW 4288423..4289565 (+) 1143 WP_060434548.1 radical SAM family heme chaperone HemW -
  F0335_RS20560 (F0335_20580) - 4289601..4289849 (-) 249 WP_149559973.1 DUF2442 domain-containing protein -
  F0335_RS20565 (F0335_20585) - 4289833..4290075 (-) 243 WP_130018804.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36459.85 Da        Isoelectric Point: 6.9307

>NTDB_id=445215 F0335_RS20525 WP_063988709.1 4284355..4285377(-) (pilT) [Serratia marcescens strain S7.1]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELHPLAAVPMLTAQGVQTLCDGLLNAQQRESLLRLGQVDLALHRPGGE
RLRANVFQQNVGMSLALRRVAGQAPSLAELAAPAIVSALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDTHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTGG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=445215 F0335_RS20525 WP_063988709.1 4284355..4285377(-) (pilT) [Serratia marcescens strain S7.1]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAACGCTTCCGATCTGCACCTTTGTGCCGGCCATCCGCCGAT
GTTGCGCATCGACGGCGAACTGCACCCGCTGGCAGCGGTGCCAATGCTGACGGCGCAAGGCGTGCAGACCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCTGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAACGTGGGGATGTCTCTCGCATTGCGGCGTGTCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTATCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACTGGTGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGACGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACACTCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGTGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAACTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCAATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACTTGA
TCCGCGAAGGAAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGTGCGGGGGAGGGAACGGGGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.455

97.059

0.538
  pilT Vibrio cholerae strain A1552

55.455

97.059

0.538
  pilT Acinetobacter baylyi ADP1

51.662

97.353

0.503
  pilT Acinetobacter baumannii D1279779

51.212

97.059

0.497
  pilT Acinetobacter nosocomialis M2

51.212

97.059

0.497
  pilT Acinetobacter baumannii strain A118

51.212

97.059

0.497
  pilT Neisseria gonorrhoeae MS11

50

98.824

0.494
  pilT Neisseria meningitidis 8013

50.602

97.647

0.494
  pilT Pseudomonas aeruginosa PAK

49.697

97.059

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.091

97.059

0.476
  pilT Legionella pneumophila strain ERS1305867

48.795

97.647

0.476
  pilT Legionella pneumophila strain Lp02

48.795

97.647

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.815

95.294

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

39.441

94.706

0.374