Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HMJ22_RS20155 Genome accession   NZ_CP053378
Coordinates   4271716..4272738 (-) Length   340 a.a.
NCBI ID   WP_171454750.1    Uniprot ID   -
Organism   Serratia marcescens strain FY     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4266716..4277738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMJ22_RS20125 - 4267529..4268041 (+) 513 WP_171454748.1 SprT family zinc-dependent metalloprotease -
  HMJ22_RS20130 endA 4268143..4268838 (+) 696 WP_085337148.1 deoxyribonuclease I -
  HMJ22_RS20135 rsmE 4268908..4269639 (+) 732 WP_171454749.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HMJ22_RS20140 gshB 4269650..4270600 (+) 951 WP_016930084.1 glutathione synthase -
  HMJ22_RS20145 - 4270734..4271297 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  HMJ22_RS20150 ruvX 4271297..4271719 (+) 423 WP_033632095.1 Holliday junction resolvase RuvX -
  HMJ22_RS20155 pilT 4271716..4272738 (-) 1023 WP_171454750.1 type IV pilus twitching motility protein PilT Machinery gene
  HMJ22_RS20160 - 4272759..4273466 (+) 708 WP_171454751.1 YggS family pyridoxal phosphate-dependent enzyme -
  HMJ22_RS20165 proC 4273486..4274307 (+) 822 WP_033632092.1 pyrroline-5-carboxylate reductase -
  HMJ22_RS20170 - 4274339..4274893 (+) 555 WP_033632091.1 YggT family protein -
  HMJ22_RS20175 yggU 4274890..4275183 (+) 294 WP_016930091.1 DUF167 family protein YggU -
  HMJ22_RS20180 - 4275324..4275917 (+) 594 WP_171454752.1 XTP/dITP diphosphatase -
  HMJ22_RS20185 hemW 4275910..4277052 (+) 1143 WP_088381924.1 radical SAM family heme chaperone HemW -
  HMJ22_RS20190 - 4277090..4277524 (-) 435 WP_019456096.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36441.85 Da        Isoelectric Point: 8.7957

>NTDB_id=444599 HMJ22_RS20155 WP_171454750.1 4271716..4272738(-) (pilT) [Serratia marcescens strain FY]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCNGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIIPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFKQG
LQQRIDAGVLGECAGEGTRG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=444599 HMJ22_RS20155 WP_171454750.1 4271716..4272738(-) (pilT) [Serratia marcescens strain FY]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTTTGCAACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGTTGGCGGCGCCGGCCATCATCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GGAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGTGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATTGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGAGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTAAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAGGGAACGAGGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Neisseria gonorrhoeae MS11

50.151

97.353

0.488
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.258

100

0.453
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376
  pilU Pseudomonas stutzeri DSM 10701

38.509

94.706

0.365