Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HMI62_RS21705 Genome accession   NZ_CP053286
Coordinates   4514379..4515401 (-) Length   340 a.a.
NCBI ID   WP_171404362.1    Uniprot ID   -
Organism   Serratia marcescens strain FZSF02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4509379..4520401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMI62_RS21675 (HMI62_21695) - 4510192..4510704 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  HMI62_RS21680 (HMI62_21700) endA 4510806..4511501 (+) 696 WP_015379043.1 deoxyribonuclease I -
  HMI62_RS21685 (HMI62_21705) rsmE 4511571..4512302 (+) 732 WP_015379044.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HMI62_RS21690 (HMI62_21710) gshB 4512313..4513263 (+) 951 WP_171404361.1 glutathione synthase -
  HMI62_RS21695 (HMI62_21715) - 4513397..4513960 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  HMI62_RS21700 (HMI62_21720) ruvX 4513960..4514382 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  HMI62_RS21705 (HMI62_21725) pilT 4514379..4515401 (-) 1023 WP_171404362.1 type IV pilus twitching motility protein PilT Machinery gene
  HMI62_RS21710 (HMI62_21730) - 4515422..4516129 (+) 708 WP_171404364.1 YggS family pyridoxal phosphate-dependent enzyme -
  HMI62_RS21715 (HMI62_21735) proC 4516149..4516970 (+) 822 WP_171404366.1 pyrroline-5-carboxylate reductase -
  HMI62_RS21720 (HMI62_21740) - 4517002..4517556 (+) 555 WP_103086314.1 YggT family protein -
  HMI62_RS21725 (HMI62_21745) yggU 4517553..4517846 (+) 294 WP_171404367.1 DUF167 family protein YggU -
  HMI62_RS21730 (HMI62_21750) - 4517987..4518580 (+) 594 WP_016930092.1 XTP/dITP diphosphatase -
  HMI62_RS21735 (HMI62_21755) hemW 4518573..4519715 (+) 1143 WP_171404368.1 radical SAM family heme chaperone HemW -
  HMI62_RS21740 (HMI62_21760) - 4519753..4520001 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36292.52 Da        Isoelectric Point: 7.2888

>NTDB_id=443885 HMI62_RS21705 WP_171404362.1 4514379..4515401(-) (pilT) [Serratia marcescens strain FZSF02]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQSRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=443885 HMI62_RS21705 WP_171404362.1 4514379..4515401(-) (pilT) [Serratia marcescens strain FZSF02]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAACAAAGCGCGGGAATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGTCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAGGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

49.849

97.353

0.485
  pilT Neisseria gonorrhoeae MS11

49.547

97.353

0.482
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Legionella pneumophila strain ERS1305867

48.795

97.647

0.476
  pilT Legionella pneumophila strain Lp02

48.795

97.647

0.476
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.503

100

0.453
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Vibrio cholerae strain A1552

39.264

95.882

0.376
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371