Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   COCOR_RS31415 Genome accession   NC_017030
Coordinates   7905058..7906170 (-) Length   370 a.a.
NCBI ID   WP_014399075.1    Uniprot ID   H8MQ54
Organism   Corallococcus coralloides DSM 2259     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7900058..7911170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COCOR_RS31400 (COCOR_06284) - 7900719..7902152 (-) 1434 WP_014399072.1 sigma-54-dependent transcriptional regulator -
  COCOR_RS31405 (COCOR_06285) - 7902149..7903717 (-) 1569 WP_014399073.1 two-component system sensor histidine kinase NtrB -
  COCOR_RS31410 (COCOR_06286) - 7903746..7905002 (-) 1257 WP_014399074.1 type II secretion system F family protein -
  COCOR_RS31415 (COCOR_06287) pilT 7905058..7906170 (-) 1113 WP_014399075.1 type IV pilus twitching motility protein PilT Machinery gene
  COCOR_RS31420 (COCOR_06288) pilB 7906219..7907919 (-) 1701 WP_014399076.1 type IV-A pilus assembly ATPase PilB -
  COCOR_RS31425 (COCOR_06289) - 7908129..7909055 (-) 927 WP_014399077.1 bifunctional riboflavin kinase/FAD synthetase -
  COCOR_RS31430 (COCOR_06290) trmB 7909137..7909757 (+) 621 WP_014399078.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  COCOR_RS31435 (COCOR_06291) - 7909932..7910975 (+) 1044 WP_043322026.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40463.65 Da        Isoelectric Point: 8.2875

>NTDB_id=44303 COCOR_RS31415 WP_014399075.1 7905058..7906170(-) (pilT) [Corallococcus coralloides DSM 2259]
MANLHQLLKAMVEKGSSDLHITTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVMSQALVAKQGAPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKYGMQTFN
QALAALLARRVISQDEAFGRSSDPEELRNILATGGGPGGAQRPAGGAGAR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=44303 COCOR_RS31415 WP_014399075.1 7905058..7906170(-) (pilT) [Corallococcus coralloides DSM 2259]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCTCTTCCGACCTCCACATCACCACCGGCTCGCCGCC
GCAGCTCCGCGTGGACGGTGAGCTCGTCCCGCTCAAGACGGCGCCGCTCACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACCTGTCGTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCGAACATCTTCATGCAGCGCGGCGCCGTCGCCGGTGCGTTCCGTACGATTCCCTTCAAGATTTTGACGTT
CCAGGAGCTGGGCCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCCGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTGAACCAGCGCGAGGTCGGTGCGGACACGCGCAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTCGTCGGCGAGTTGCGCGACCTGGAGACCATCGAAG
CGGCGCTCACCATCGCGGAGACAGGCCACATCTGCTACGCGACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
ATCCTGGACGTCTTCCCCCCGTACCAGCAGCCCCAGGTGCGCGCCCAGCTGTCCTTCGTGCTGGAAGGCGTGATGAGCCA
GGCGCTCGTCGCCAAGCAGGGCGCTCCGGGCCGCGTGCTGGCCCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTGGGTCAGGCGAAGTACGGCATGCAGACGTTCAAC
CAGGCGCTGGCCGCCCTTCTGGCGCGCCGGGTCATCTCCCAGGACGAGGCCTTCGGCCGTTCTTCCGACCCGGAGGAGCT
GCGCAACATCCTCGCCACGGGCGGTGGACCGGGTGGAGCCCAGCGGCCGGCCGGGGGAGCGGGTGCCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H8MQ54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.378

0.492
  pilT Legionella pneumophila strain ERS1305867

54.03

90.541

0.489
  pilT Legionella pneumophila strain Lp02

54.03

90.541

0.489
  pilT Pseudomonas stutzeri DSM 10701

51.014

93.243

0.476
  pilT Pseudomonas aeruginosa PAK

51.014

93.243

0.476
  pilT Acinetobacter baylyi ADP1

51.014

93.243

0.476
  pilT Acinetobacter baumannii D1279779

51.014

93.243

0.476
  pilT Acinetobacter baumannii strain A118

51.014

93.243

0.476
  pilT Acinetobacter nosocomialis M2

50.725

93.243

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.102

90.27

0.443
  pilT Vibrio cholerae strain A1552

49.102

90.27

0.443
  pilT Neisseria meningitidis 8013

44.575

92.162

0.411
  pilT Neisseria gonorrhoeae MS11

44.575

92.162

0.411
  pilU Vibrio cholerae strain A1552

43.059

95.405

0.411
  pilU Pseudomonas stutzeri DSM 10701

41.011

96.216

0.395
  pilU Acinetobacter baylyi ADP1

40.346

93.784

0.378

Multiple sequence alignment