Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HKT17_RS11010 Genome accession   NZ_CP053084
Coordinates   2350025..2351059 (-) Length   344 a.a.
NCBI ID   WP_105029633.1    Uniprot ID   A0A520TJ09
Organism   Limnobacter sp. SAORIC-580     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2345025..2356059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKT17_RS10985 (HKT17_10990) holA 2345295..2346335 (+) 1041 WP_171100074.1 DNA polymerase III subunit delta -
  HKT17_RS10990 (HKT17_10995) - 2346372..2347628 (+) 1257 WP_171100076.1 glutamate-5-semialdehyde dehydrogenase -
  HKT17_RS10995 (HKT17_11000) - 2347656..2348087 (+) 432 WP_171101494.1 CopD family protein -
  HKT17_RS11000 (HKT17_11005) - 2348105..2349466 (+) 1362 WP_171100077.1 class I SAM-dependent RNA methyltransferase -
  HKT17_RS11005 (HKT17_11010) - 2349479..2349967 (-) 489 WP_105029632.1 glutathione peroxidase -
  HKT17_RS11010 (HKT17_11015) pilT 2350025..2351059 (-) 1035 WP_105029633.1 type IV pilus twitching motility protein PilT Machinery gene
  HKT17_RS11015 (HKT17_11020) - 2351099..2351812 (+) 714 WP_171100080.1 YggS family pyridoxal phosphate-dependent enzyme -
  HKT17_RS11020 (HKT17_11025) proC 2351828..2352634 (+) 807 WP_171100082.1 pyrroline-5-carboxylate reductase -
  HKT17_RS11025 (HKT17_11030) ubiA 2352664..2353527 (-) 864 WP_171100083.1 4-hydroxybenzoate octaprenyltransferase -
  HKT17_RS11030 (HKT17_11035) - 2353531..2354475 (-) 945 WP_171100085.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37907.45 Da        Isoelectric Point: 6.6368

>NTDB_id=442905 HKT17_RS11010 WP_105029633.1 2350025..2351059(-) (pilT) [Limnobacter sp. SAORIC-580]
MTLTELLTWAVEEHASDLHLSADMPPMIRVNGEMRKLSESVLGHREIYRLIQGSMTEAQWQGWEQSHECDYSFSVDGLSR
FRVNAFMQSRGASAAFRVVPSRVKTLEELEAPAVFRQIAREPNGLVLVTGPTGSGKSTTLAAMVDDVNRNQKAHILTIED
PIEFTHTPMNCVINQRELGEHTHSFGAALRSALREDPDVILVGEMRDLETIQLALTAAETGHLVFATLHTSSAPKTIDRI
IDAFPSADKSMVRAMLSESLRAVICQTLLKKQGGGRVAAHEIMMATPPIRNLIREGKVAQMLSSIQTGSQHGMQTMEQAV
QRLLMTGRITREVAMSVISGSNQP

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=442905 HKT17_RS11010 WP_105029633.1 2350025..2351059(-) (pilT) [Limnobacter sp. SAORIC-580]
ATGACACTAACCGAGTTGTTAACCTGGGCTGTTGAAGAGCATGCATCGGATTTGCATTTGTCGGCAGACATGCCACCGAT
GATCCGTGTAAACGGTGAAATGCGCAAACTGAGTGAATCTGTGTTGGGACACCGTGAGATTTATCGACTGATTCAGGGGT
CGATGACAGAAGCGCAATGGCAAGGCTGGGAACAGTCGCATGAATGTGATTACAGTTTTTCAGTGGATGGTTTGTCGCGT
TTTCGTGTGAATGCATTCATGCAGAGCAGGGGTGCATCGGCGGCATTTCGGGTGGTGCCCAGCCGAGTAAAAACGCTGGA
GGAACTGGAGGCACCCGCCGTGTTCCGTCAAATTGCGCGTGAACCCAATGGTCTGGTACTGGTTACCGGTCCCACCGGCT
CGGGCAAAAGTACCACCTTGGCAGCGATGGTGGATGATGTAAACCGCAACCAGAAGGCGCATATTCTGACCATTGAGGAC
CCGATTGAATTTACGCACACACCGATGAACTGTGTGATTAACCAACGCGAGTTGGGTGAGCACACCCATTCATTTGGTGC
AGCTTTGCGTTCTGCCCTGCGAGAGGACCCAGATGTAATTCTGGTTGGCGAAATGCGTGATCTTGAAACCATTCAACTGG
CCTTGACTGCAGCTGAAACCGGGCACCTGGTTTTTGCCACCTTGCACACTTCCTCTGCACCCAAAACCATTGACCGTATT
ATTGATGCATTCCCATCGGCTGACAAAAGCATGGTGCGCGCCATGTTGTCGGAATCGCTGCGTGCCGTAATTTGTCAAAC
CCTGCTGAAAAAGCAGGGGGGCGGGCGAGTTGCTGCGCATGAAATCATGATGGCCACGCCGCCGATTCGAAATTTGATTC
GTGAAGGCAAGGTGGCGCAAATGTTGTCGTCCATTCAAACGGGTTCGCAGCATGGCATGCAGACCATGGAGCAGGCGGTG
CAACGGCTTTTGATGACTGGAAGGATTACTCGAGAAGTGGCGATGAGTGTGATTTCGGGCTCGAACCAGCCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A520TJ09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

64.095

97.965

0.628
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.905

98.256

0.628
  pilT Vibrio cholerae strain A1552

63.905

98.256

0.628
  pilT Acinetobacter baumannii strain A118

62.874

97.093

0.61
  pilT Acinetobacter nosocomialis M2

62.874

97.093

0.61
  pilT Acinetobacter baumannii D1279779

62.874

97.093

0.61
  pilT Pseudomonas stutzeri DSM 10701

62.575

97.093

0.608
  pilT Pseudomonas aeruginosa PAK

61.677

97.093

0.599
  pilT Legionella pneumophila strain Lp02

59.281

97.093

0.576
  pilT Legionella pneumophila strain ERS1305867

59.281

97.093

0.576
  pilT Neisseria meningitidis 8013

58.806

97.384

0.573
  pilT Neisseria gonorrhoeae MS11

58.209

97.384

0.567
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.214

97.674

0.471
  pilU Pseudomonas stutzeri DSM 10701

40.525

99.709

0.404
  pilU Vibrio cholerae strain A1552

38.484

99.709

0.384
  pilU Acinetobacter baylyi ADP1

38.509

93.605

0.36