Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DSM104440_RS00685 Genome accession   NZ_CP053073
Coordinates   139957..141000 (-) Length   347 a.a.
NCBI ID   WP_171159805.1    Uniprot ID   A0A6M4H2R0
Organism   Usitatibacter palustris strain Swamp67     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 134957..146000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM104440_RS19345 (DSM104440_00126) - 135287..135661 (-) 375 WP_171159793.1 hypothetical protein -
  DSM104440_RS00655 - 135843..136247 (-) 405 WP_171159795.1 hypothetical protein -
  DSM104440_RS00665 - 136791..137096 (-) 306 WP_171159797.1 hypothetical protein -
  DSM104440_RS00675 xerC 137691..138704 (+) 1014 WP_171159801.1 site-specific tyrosine recombinase XerC -
  DSM104440_RS00680 (DSM104440_00130) pilU 138807..139946 (-) 1140 WP_171159803.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DSM104440_RS00685 (DSM104440_00131) pilT 139957..141000 (-) 1044 WP_171159805.1 type IV pilus twitching motility protein PilT Machinery gene
  DSM104440_RS00690 (DSM104440_00132) - 141047..141760 (+) 714 WP_171159807.1 YggS family pyridoxal phosphate-dependent enzyme -
  DSM104440_RS00695 (DSM104440_00133) proC 141757..142578 (+) 822 WP_171159809.1 pyrroline-5-carboxylate reductase -
  DSM104440_RS00700 (DSM104440_00134) - 142575..143162 (+) 588 WP_171159811.1 YggT family protein -
  DSM104440_RS00705 (DSM104440_00135) - 143263..144945 (-) 1683 WP_212758156.1 long-chain fatty acid--CoA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38296.13 Da        Isoelectric Point: 6.9357

>NTDB_id=442730 DSM104440_RS00685 WP_171159805.1 139957..141000(-) (pilT) [Usitatibacter palustris strain Swamp67]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIPALEHKDVHGMVYDIMNDSQRKVYEETLEVDFSFEIPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNAPKIFAEIANQPRGLVLVTGPTGSGKSTTLAAMVNHVNEHEYGHILTVED
PIEFVHESKKCLMNQREVGPHTLSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAAEKEMIRAMLSESLRSVISQTLCKTKDGTGRVAAHEIMIGTPAIRNLIREAKVAQMYSSIQTGQALGMQTLDQN
LLDLVKRNLVAAPEARNKAMNKDLFLG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=442730 DSM104440_RS00685 WP_171159805.1 139957..141000(-) (pilT) [Usitatibacter palustris strain Swamp67]
ATGGACATTGCTGAACTGCTCGCCTTTTCGGTCAAGAACAAAGCCTCGGACCTGCACCTTTCCGCGGGCCTGCCTCCCAT
GATCCGCGTGCACGGCGACGTGCGGCGCATCAACATCCCCGCGCTCGAGCACAAGGACGTGCACGGGATGGTCTACGACA
TCATGAACGACTCCCAGCGCAAGGTTTACGAAGAGACGCTGGAAGTGGACTTCTCGTTCGAGATCCCGAACCTCGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCGGGTGCTGTGTTCCGGACGATTCCTTCCAAGGTGCTGACGCTCGA
GGAGCTCAACGCGCCGAAGATCTTCGCGGAAATCGCGAACCAGCCGCGCGGCCTCGTGCTCGTCACGGGCCCGACGGGCT
CGGGCAAGTCGACGACGCTCGCGGCGATGGTGAACCACGTGAACGAACACGAGTACGGCCACATCCTCACGGTCGAGGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGATGAACCAGCGCGAGGTCGGACCCCACACGCTCTCGTTCTCGAA
CGCGCTGCGCAGCGCGCTTCGCGAGGACCCGGACATCATCCTCGTGGGCGAAATGCGCGACCTGGAAACCATTCGCCTCG
CGATGACGGCCGCGGAAACGGGCCACCTGGTGTTCGGCACGCTGCATACGTCGTCCGCGGCGAAGACCGTGGACCGCATC
ATCGACGTGTTCCCCGCGGCGGAAAAGGAAATGATCCGCGCGATGTTGTCGGAGTCCCTGCGCTCCGTGATCTCGCAGAC
ACTGTGCAAGACCAAGGACGGCACGGGCCGCGTCGCCGCCCACGAAATCATGATCGGCACGCCCGCCATCCGGAACCTCA
TCCGCGAGGCGAAGGTCGCACAGATGTATTCATCCATCCAGACCGGCCAGGCGCTGGGCATGCAGACACTCGACCAGAAC
CTGCTCGACCTTGTGAAGCGCAATCTTGTCGCGGCCCCCGAGGCCCGGAACAAGGCGATGAACAAGGACCTCTTCCTCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M4H2R0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.59

99.712

0.764
  pilT Acinetobacter baumannii D1279779

76.59

99.712

0.764
  pilT Acinetobacter baumannii strain A118

76.59

99.712

0.764
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

77.286

97.695

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

73.623

99.424

0.732
  pilT Legionella pneumophila strain ERS1305867

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

70.554

98.847

0.697
  pilT Neisseria gonorrhoeae MS11

70.262

98.847

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.71

97.406

0.689
  pilT Vibrio cholerae strain A1552

70.71

97.406

0.689
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

42.183

97.695

0.412
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.708

97.695

0.398